The L17 ribosomal protein of Bacillus subtilis binds preferentially to curved DNA

We searched in Bacillus subtilis for proteins that bind preferentially to curved DNA. Two proteins of 9 and 15 kDa displaying this property were purified from exponentially growing cells of B. subtilis strain 168. Sequencing of N-terminal amino acids identified them as the proteins HBsu and L17 resp...

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Veröffentlicht in:	Biochimie 2000, Vol.82 (1), p.85-91
Hauptverfasser:	Zouine, M., Beloin, C., Ghelis, C., Le Hégarat, F.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacillus subtilis Bacillus subtilis - genetics Bacillus subtilis - metabolism Bacterial Proteins Cell Division - genetics curved DNA DNA, Bacterial - chemistry DNA, Bacterial - metabolism DNA-Binding Proteins - isolation & purification DNA-Binding Proteins - metabolism Escherichia coli Escherichia coli - genetics Escherichia coli - growth & development HBsu L17 Life Sciences Recombinant Proteins - genetics Recombinant Proteins - metabolism Ribosomal Proteins - metabolism
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Beschreibung
Zusammenfassung:	We searched in Bacillus subtilis for proteins that bind preferentially to curved DNA. Two proteins of 9 and 15 kDa displaying this property were purified from exponentially growing cells of B. subtilis strain 168. Sequencing of N-terminal amino acids identified them as the proteins HBsu and L17 respectively. The overproduction of L17 from B. subtilis in Escherichia coli was shown to have a strong effect on nucleoid morphology and segregation.
ISSN:	0300-9084 1638-6183
DOI:	10.1016/S0300-9084(00)00184-X