Variation in copy number of the 28S rDNA of Aspergillus fumigatus measured by droplet digital PCR and analog quantitative real-time PCR

Droplet digital PCR (ddPCR) after DNA digestion yielded a 28S rDNA copy number of 61 to 86 copies/genome when testing 10 unrelated Aspergillus fumigatus isolates, higher than with quantitative PCR. Unfortunately, ddPCR after DNA digestion did not improve the sensitivity of our PCR assay when testing...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of microbiological methods 2016-08, Vol.127, p.160-163
Hauptverfasser:	Alanio, Alexandre, Sturny-Leclère, Aude, Benabou, Marion, Guigue, Nicolas, Bretagne, Stéphane
Format:	Artikel
Sprache:	eng
Schlagworte:	Aspergillosis - diagnosis Aspergillosis - microbiology Aspergillus fumigatus Aspergillus fumigatus - genetics Biochemistry, Molecular Biology Copy number variation DNA Copy Number Variations DNA, Ribosomal - genetics DNA, Ribosomal - isolation & purification Droplet digital PCR Humans Life Sciences Microbiology and Parasitology Molecular biology Mycology rDNA Real-Time Polymerase Chain Reaction - instrumentation Real-Time Polymerase Chain Reaction - methods Real-time quantitative PCR RNA, Ribosomal, 28S - genetics Sensitivity and Specificity
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Droplet digital PCR (ddPCR) after DNA digestion yielded a 28S rDNA copy number of 61 to 86 copies/genome when testing 10 unrelated Aspergillus fumigatus isolates, higher than with quantitative PCR. Unfortunately, ddPCR after DNA digestion did not improve the sensitivity of our PCR assay when testing serum patients with invasive aspergillosis.
ISSN:	0167-7012 1872-8359
DOI:	10.1016/j.mimet.2016.06.015