Vasopressin, ATP and catecholamines differentially control potassium secretion in inner ear cell line

► Inner ear EC5v cells express vasopressin, β-adrenergic, and purinergic receptors. ► Adenylate cyclase, phosphoinositidase C, and Ca 2+ signaling pathways are functional. ► Vasopressin and β agonists stimulate, and ATP inhibits apical potassium secretion. ► Endolymph homeostasis is maintained by GP...

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Veröffentlicht in:	FEBS letters 2011-09, Vol.585 (17), p.2703-2708
Hauptverfasser:	Nevoux, Jérôme, Teixeira, Marie, Viengchareun, Say, Cosson, Claudine, Butlen, Daniel, Lombès, Marc, Ferrary, Evelyne
Format:	Artikel
Sprache:	eng
Schlagworte:	1-deamino-8d-arginine vasopressin 2′- and 3′-0-4-(benzoyl benzyl)-ATP [1-(β-mercapto-β,β-cyclopentamethylene propionic acid), 2-0-ethyltyrosine, 4-valine, 8-arginiamide] vasopressin [desgly9-d (CH2)5-Tyr (Et)2 VAVP] adenosine 5′-0-(3-thriophosphate) Adenosine Triphosphate Adenosine Triphosphate - pharmacology Adenylate Cyclase Adenylyl Cyclases - metabolism Angiotensin II Animals Arginine Vasopressin Asn1, Val5-angiotensin II ATP ATPγS AVP Blotting, Western Bovine Serum Albumin BSA Bz-ATP Calcium Signaling Calcium Signaling - drug effects cAMP Catecholamines Catecholamines - pharmacology CCD Cell Line Cortical Collecting Duct cyclic Adenosine Monophosphate Cyclic AMP Cyclic AMP - metabolism dDAVP desGly Ear, Inner Ear, Inner - cytology Ear, Inner - drug effects Ear, Inner - metabolism Endolymph Forskoline FSK G Protein Coupled Receptors Glycerophosphoinositol GPCR GroPns hPTH Human health and pathology human parathormone Inositol Phosphates InsPs Life Sciences Menière's disease Mice Ornithine Vasotocin Analogue OVTA PGE2 Phe2, Orn8- vasotocin: [Phe2, Orn8]VT Phosphoinositidase C Phosphoric Diester Hydrolases Phosphoric Diester Hydrolases - metabolism PLC Potassium Potassium - metabolism poVT Prostaglandin E2 Purinergic receptors Receptors, Purinergic P2Y Receptors, Purinergic P2Y - metabolism Receptors, Vasopressin Receptors, Vasopressin - metabolism Reverse Transcriptase Polymerase Chain Reaction Sensory Organs total inositol phosphates UDP UMP Uridine Diphosphate Uridine Monophosphate Uridine Triphosphate UTP V2R Vasopressin receptors Vasopressin type 2 receptor Vasopressins Vasopressins - pharmacology Vestibular cells α,β-Me-ATP α,β-methylene-ATP β,γ-Me-ATP β,γ-methylene ATP ΣInsPs
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Zusammenfassung:	► Inner ear EC5v cells express vasopressin, β-adrenergic, and purinergic receptors. ► Adenylate cyclase, phosphoinositidase C, and Ca 2+ signaling pathways are functional. ► Vasopressin and β agonists stimulate, and ATP inhibits apical potassium secretion. ► Endolymph homeostasis is maintained by GPCR-activated hormones. ► This opens new therapeutic avenues in Menière’s disease. A strict control of endolymph composition (high potassium, low sodium fluid) and volume is instrumental for a proper functioning of the inner ear. Alteration of endolymph homeostasis is proposed in the pathogenesis of Menière’s disease. However, the mechanisms controlling endolymph secretion remain elusive. By using the vestibular EC5v cells, we provide evidence for the presence of vasopressin, catecholamine and purinergic signaling pathways, coupled to adenylate cyclase, phosphoinositidase C and Ca 2+ activation. We demonstrate that vasopressin and catecholamines stimulate while ATP inhibits apical potassium secretion by EC5v cells. These results open new interesting perspectives for the management of inner ear diseases.
ISSN:	0014-5793 1873-3468
DOI:	10.1016/j.febslet.2011.07.025