Comment on "Obestatin, a peptide encoded by the ghrelin gene, opposes ghrelin's effects on food intake"

Zhang et al. (Research Articles, 11 November 2005, p. 996) reported that obestatin, a peptide derived from the ghrelin precursor, activated the orphan G protein-coupled receptor GPR39. However, we found that I125-obestatin does not bind GPR39 and observed no effects of obestatin on GPR39-transfected...

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Veröffentlicht in:	Science (American Association for the Advancement of Science) 2007-02, Vol.315 (5813), p.766; author reply 766-766
Hauptverfasser:	Chartrel, N, Alvear-Perez, R, Leprince, J, Iturrioz, X, Reaux-Le Goazigo, A, Audinot, V, Chomarat, P, Coge, F, Nosjean, O, Rodriguez, M, Galizzi, J P, Boutin, J A, Vaudry, H, Llorens-Cortes, C
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Base Sequence Calcium Calcium - metabolism Cell Membrane Cell Membrane - metabolism CHO Cells Colforsin - pharmacology Cricetinae Cricetulus Cyclic AMP Cyclic AMP - metabolism Endocrinology and metabolism Forskolin Ghrelin Human health and pathology Humans Life Sciences Ligands Molecular Sequence Data Peptide Hormones Peptide Hormones - genetics Peptide Hormones - metabolism Peptide Hormones - pharmacology Pituitary Gland Pituitary Gland - cytology Pituitary Gland - metabolism Protein Binding Receptors, G-Protein-Coupled Receptors, G-Protein-Coupled - genetics Receptors, G-Protein-Coupled - metabolism Transfection
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Beschreibung
Zusammenfassung:	Zhang et al. (Research Articles, 11 November 2005, p. 996) reported that obestatin, a peptide derived from the ghrelin precursor, activated the orphan G protein-coupled receptor GPR39. However, we found that I125-obestatin does not bind GPR39 and observed no effects of obestatin on GPR39-transfected cells in various functional assays (cyclic adenosine monophosphate production, calcium mobilization, and GPR39 internalization). Our results indicate that obestatin is not the cognate ligand for GPR39.
ISSN:	0036-8075 1095-9203
DOI:	10.1126/science.1135047