Anti-androgenic activities of environmental pesticides in the MDA-kb2 reporter cell line
Pesticides have been suspected to act as endocrine disruptive compounds (EDCs) through several mechanisms of action, however data are still needed for a number of currently used pesticides. In the present study, 30 environmental pesticides selected from different chemical classes (azole, carbamate,...
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| Veröffentlicht in: | Toxicology in vitro 2010-10, Vol.24 (7), p.1979-1985 |
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| container_issue | 7 |
| container_start_page | 1979 |
| container_title | Toxicology in vitro |
| container_volume | 24 |
| creator | Aït-Aïssa, S. Laskowski, S. Laville, N. Porcher, J.-M. Brion, F. |
| description | Pesticides have been suspected to act as endocrine disruptive compounds (EDCs) through several mechanisms of action, however data are still needed for a number of currently used pesticides. In the present study, 30 environmental pesticides selected from different chemical classes (azole, carbamate, dicarboximide, organochlorine, organophosphorus, oxadiazole, phenylureas, pyrazole, pyrimidine, pyrethroid and sulfonylureas) were tested for their ability to alter
in vitro the transcriptional activity of the androgen receptor in the MDA-kb2 reporter cell line. The responsiveness of the system was checked by using a panel of reference ligands of androgen and glucocorticoid receptors. When tested alone at concentrations up to 10
μM, none of the studied pesticides were able to induce the reporter gene after a 18
h exposure. Conversely, co-exposure experiments with 0.1
nM dihydrotestosterone (DHT) allowed identifying 15 active pesticides with IC
50 ranging from 0.2
μM for vinclozolin to 12
μM for fenarimol. Fipronil and bupirimate were here newly described for their AR antagonistic activity. |
| doi_str_mv | 10.1016/j.tiv.2010.08.014 |
| format | Article |
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in vitro the transcriptional activity of the androgen receptor in the MDA-kb2 reporter cell line. The responsiveness of the system was checked by using a panel of reference ligands of androgen and glucocorticoid receptors. When tested alone at concentrations up to 10
μM, none of the studied pesticides were able to induce the reporter gene after a 18
h exposure. Conversely, co-exposure experiments with 0.1
nM dihydrotestosterone (DHT) allowed identifying 15 active pesticides with IC
50 ranging from 0.2
μM for vinclozolin to 12
μM for fenarimol. Fipronil and bupirimate were here newly described for their AR antagonistic activity.</description><identifier>ISSN: 0887-2333</identifier><identifier>EISSN: 1879-3177</identifier><identifier>DOI: 10.1016/j.tiv.2010.08.014</identifier><identifier>PMID: 20736058</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Androgen receptor ; Androgen Receptor Antagonists - administration & dosage ; Androgen Receptor Antagonists - toxicity ; Androgen receptors ; Anti-androgenic potency ; Cell Line, Tumor ; Dihydrotestosterone - toxicity ; Endocrine Disruptors - administration & dosage ; Endocrine Disruptors - toxicity ; Genes, Reporter - drug effects ; Glucocorticoid receptor ; Humans ; Inhibitory Concentration 50 ; Life Sciences ; Pesticides ; Pesticides - toxicity ; Reporter gene assay ; Time Factors ; Toxicology ; Transcription, Genetic - drug effects</subject><ispartof>Toxicology in vitro, 2010-10, Vol.24 (7), p.1979-1985</ispartof><rights>2010 Elsevier Ltd</rights><rights>Copyright © 2010 Elsevier Ltd. All rights reserved.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c530t-1185e1144c9393736eafe90521f271b050730e9946940dd6175add2167315b1f3</citedby><cites>FETCH-LOGICAL-c530t-1185e1144c9393736eafe90521f271b050730e9946940dd6175add2167315b1f3</cites><orcidid>0000-0003-2341-4196</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.tiv.2010.08.014$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3541,27915,27916,45986</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20736058$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://ineris.hal.science/ineris-00511869$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Aït-Aïssa, S.</creatorcontrib><creatorcontrib>Laskowski, S.</creatorcontrib><creatorcontrib>Laville, N.</creatorcontrib><creatorcontrib>Porcher, J.-M.</creatorcontrib><creatorcontrib>Brion, F.</creatorcontrib><title>Anti-androgenic activities of environmental pesticides in the MDA-kb2 reporter cell line</title><title>Toxicology in vitro</title><addtitle>Toxicol In Vitro</addtitle><description>Pesticides have been suspected to act as endocrine disruptive compounds (EDCs) through several mechanisms of action, however data are still needed for a number of currently used pesticides. In the present study, 30 environmental pesticides selected from different chemical classes (azole, carbamate, dicarboximide, organochlorine, organophosphorus, oxadiazole, phenylureas, pyrazole, pyrimidine, pyrethroid and sulfonylureas) were tested for their ability to alter
in vitro the transcriptional activity of the androgen receptor in the MDA-kb2 reporter cell line. The responsiveness of the system was checked by using a panel of reference ligands of androgen and glucocorticoid receptors. When tested alone at concentrations up to 10
μM, none of the studied pesticides were able to induce the reporter gene after a 18
h exposure. Conversely, co-exposure experiments with 0.1
nM dihydrotestosterone (DHT) allowed identifying 15 active pesticides with IC
50 ranging from 0.2
μM for vinclozolin to 12
μM for fenarimol. Fipronil and bupirimate were here newly described for their AR antagonistic activity.</description><subject>Androgen receptor</subject><subject>Androgen Receptor Antagonists - administration & dosage</subject><subject>Androgen Receptor Antagonists - toxicity</subject><subject>Androgen receptors</subject><subject>Anti-androgenic potency</subject><subject>Cell Line, Tumor</subject><subject>Dihydrotestosterone - toxicity</subject><subject>Endocrine Disruptors - administration & dosage</subject><subject>Endocrine Disruptors - toxicity</subject><subject>Genes, Reporter - drug effects</subject><subject>Glucocorticoid receptor</subject><subject>Humans</subject><subject>Inhibitory Concentration 50</subject><subject>Life Sciences</subject><subject>Pesticides</subject><subject>Pesticides - toxicity</subject><subject>Reporter gene assay</subject><subject>Time Factors</subject><subject>Toxicology</subject><subject>Transcription, Genetic - drug effects</subject><issn>0887-2333</issn><issn>1879-3177</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFvEzEQhS0EoqHwA7igvXFhw4y9XtviFLXQIgVxAYmb5XhnqcPGG2wnEv8eR2l7hJM18jdv3sxj7DXCEgH799tlCcclh1qDXgJ2T9gCtTKtQKWesgVorVouhLhgL3LeAoDUHJ6zCw5K9LVYsB-rWELr4pDmnxSDb5yvmqEEys08NhSPIc1xR7G4qdlTLsGHof6F2JQ7ar5cr9pfG94k2s-pUGo8TVMzhUgv2bPRTZle3b-X7Punj9-ubtv115vPV6t166WA0iJqSYhd540woroiN5IByXHkCjcgq1MgY7redDAMPSrphoFjrwTKDY7ikr076965ye5T2Ln0x84u2NvV2lYfKWRb965zenPEir894_s0_z7Ufewu5JNpF2k-ZKtVh6qeTfyXVFKa3nANlcQz6dOcc6Lx0QeCPQVlt7Ye1Z6CsqBtDar2vLlXP2x2NDx2PCRTgQ9ngOrxjoGSzT5Q9DSERL7YYQ7_kP8LJGKhIQ</recordid><startdate>20101001</startdate><enddate>20101001</enddate><creator>Aït-Aïssa, S.</creator><creator>Laskowski, S.</creator><creator>Laville, N.</creator><creator>Porcher, J.-M.</creator><creator>Brion, F.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7U7</scope><scope>C1K</scope><scope>1XC</scope><scope>VOOES</scope><orcidid>https://orcid.org/0000-0003-2341-4196</orcidid></search><sort><creationdate>20101001</creationdate><title>Anti-androgenic activities of environmental pesticides in the MDA-kb2 reporter cell line</title><author>Aït-Aïssa, S. ; 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In the present study, 30 environmental pesticides selected from different chemical classes (azole, carbamate, dicarboximide, organochlorine, organophosphorus, oxadiazole, phenylureas, pyrazole, pyrimidine, pyrethroid and sulfonylureas) were tested for their ability to alter
in vitro the transcriptional activity of the androgen receptor in the MDA-kb2 reporter cell line. The responsiveness of the system was checked by using a panel of reference ligands of androgen and glucocorticoid receptors. When tested alone at concentrations up to 10
μM, none of the studied pesticides were able to induce the reporter gene after a 18
h exposure. Conversely, co-exposure experiments with 0.1
nM dihydrotestosterone (DHT) allowed identifying 15 active pesticides with IC
50 ranging from 0.2
μM for vinclozolin to 12
μM for fenarimol. Fipronil and bupirimate were here newly described for their AR antagonistic activity.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>20736058</pmid><doi>10.1016/j.tiv.2010.08.014</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0003-2341-4196</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Toxicology in vitro, 2010-10, Vol.24 (7), p.1979-1985 |
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| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE |
| subjects | Androgen receptor Androgen Receptor Antagonists - administration & dosage Androgen Receptor Antagonists - toxicity Androgen receptors Anti-androgenic potency Cell Line, Tumor Dihydrotestosterone - toxicity Endocrine Disruptors - administration & dosage Endocrine Disruptors - toxicity Genes, Reporter - drug effects Glucocorticoid receptor Humans Inhibitory Concentration 50 Life Sciences Pesticides Pesticides - toxicity Reporter gene assay Time Factors Toxicology Transcription, Genetic - drug effects |
| title | Anti-androgenic activities of environmental pesticides in the MDA-kb2 reporter cell line |
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