Anti-androgenic activities of environmental pesticides in the MDA-kb2 reporter cell line
Pesticides have been suspected to act as endocrine disruptive compounds (EDCs) through several mechanisms of action, however data are still needed for a number of currently used pesticides. In the present study, 30 environmental pesticides selected from different chemical classes (azole, carbamate,...
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Veröffentlicht in: | Toxicology in vitro 2010-10, Vol.24 (7), p.1979-1985 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Pesticides have been suspected to act as endocrine disruptive compounds (EDCs) through several mechanisms of action, however data are still needed for a number of currently used pesticides. In the present study, 30 environmental pesticides selected from different chemical classes (azole, carbamate, dicarboximide, organochlorine, organophosphorus, oxadiazole, phenylureas, pyrazole, pyrimidine, pyrethroid and sulfonylureas) were tested for their ability to alter
in vitro the transcriptional activity of the androgen receptor in the MDA-kb2 reporter cell line. The responsiveness of the system was checked by using a panel of reference ligands of androgen and glucocorticoid receptors. When tested alone at concentrations up to 10
μM, none of the studied pesticides were able to induce the reporter gene after a 18
h exposure. Conversely, co-exposure experiments with 0.1
nM dihydrotestosterone (DHT) allowed identifying 15 active pesticides with IC
50 ranging from 0.2
μM for vinclozolin to 12
μM for fenarimol. Fipronil and bupirimate were here newly described for their AR antagonistic activity. |
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ISSN: | 0887-2333 1879-3177 |
DOI: | 10.1016/j.tiv.2010.08.014 |