Differential Binding Regulation of Microtubule-associated Proteins MAP1A, MAP1B, and MAP2 by Tubulin Polyglutamylation
The major neuronal post-translational modification of tubulin, polyglutamylation, can act as a molecular potentiometer to modulate microtubule-associated proteins (MAPs) binding as a function of the polyglutamyl chain length. The relative affinity of Tau, MAP2, and kinesin has been shown to be optim...
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Veröffentlicht in: | The Journal of biological chemistry 2001-04, Vol.276 (16), p.12839-12848 |
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Zusammenfassung: | The major neuronal post-translational modification of tubulin, polyglutamylation, can act as a molecular potentiometer to
modulate microtubule-associated proteins (MAPs) binding as a function of the polyglutamyl chain length. The relative affinity
of Tau, MAP2, and kinesin has been shown to be optimal for tubulin modified by â¼3 glutamyl units. Using blot overlay assays,
we have tested the ability of polyglutamylation to modulate the interaction of two other structural MAPs, MAP1A and MAP1B,
with tubulin. MAP1A and MAP2 display distinct behavior in terms of tubulin binding; they do not compete with each other, even
when the polyglutamyl chains of tubulin are removed, indicating that they have distinct binding sites on tubulin. Binding
of MAP1A and MAP1B to tubulin is also controlled by polyglutamylation and, although the modulation of MAP1B binding resembles
that of MAP2, we found that polyglutamylation can exert a different mode of regulation toward MAP1A. Interestingly, although
the affinity of the other MAPs tested so far decreases sharply for tubulins carrying long polyglutamyl chains, the affinity
of MAP1A for these tubulins is maintained at a significant level. This differential regulation exerted by polyglutamylation
toward different MAPs might facilitate their selective recruitment into distinct microtubule populations, hence modulating
their functional properties. |
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ISSN: | 0021-9258 1083-351X |
DOI: | 10.1074/jbc.M011380200 |