Determination of citrulline in human plasma, red blood cells and urine by electron impact (EI) ionization gas chromatography–mass spectrometry

A method was developed by using gas chromatography–mass spectrometry in the electron impact ionization mode to quantify citrulline in plasma, red blood cells (RBC) and urine. For all three fluids, citrulline was extracted on ion exchange resins, before derivatization to its propyl-heptaflorobutyryl-...

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Veröffentlicht in:	Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2008-04, Vol.865 (1), p.40-47
Hauptverfasser:	Rougé, Carole, Des Robert, Clotilde, Robins, Alexander, Le Bacquer, Olivier, De La Cochetière, Marie-France, Darmaun, Dominique
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino acids Analysis Analytical, structural and metabolic biochemistry Biological and medical sciences Calibration Citrulline - blood Erythrocytes - chemistry Fundamental and applied biological sciences. Psychology Gas Chromatography-Mass Spectrometry - methods General pharmacology Humans Life Sciences Medical sciences Nutrition Pharmacology. Drug treatments Protein Reproducibility of Results Spectrometry, Mass, Electrospray Ionization - methods Stable isotopes Urine - chemistry
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Beschreibung
Zusammenfassung:	A method was developed by using gas chromatography–mass spectrometry in the electron impact ionization mode to quantify citrulline in plasma, red blood cells (RBC) and urine. For all three fluids, citrulline was extracted on ion exchange resins, before derivatization to its propyl-heptaflorobutyryl-ester. Assay precision (coefficient of variation, CV) was 90% and the within- and between-day CV were
ISSN:	1570-0232 1873-376X
DOI:	10.1016/j.jchromb.2008.01.055