Emissions of volatile organic compounds from aboveground and belowground parts of rapeseed (Brassica napus L.) and tomato (Solanum lycopersicum L.)

Root systems represent a source of Volatile Organic Compounds (VOCs) that may significantly contribute to the atmospheric VOC emissions from agroecosystems and shape soil microbial activity. To gain deeper insights into the role of roots in the VOC emissions from crops, we developed a dynamic chamber with isolated aboveground and belowground compartments, allowing for simultaneous measurements of VOC fluxes from both compartments in controlled conditions. We continuously monitored VOC emissions from intact plants of rapeseed (Brassica napus L.) and tomato (Solanum lycopersicum L.) i) over 24 h when plants were rooted in soil, and ii) over 6 h following soil removal. The measurements were performed using a highly sensitive Proton Transfer Reaction – Time of Flight – Mass Spectrometer and a Thermic Desorption- Gas Chromatography – Mass Spectrometer. Net VOC emissions measured at the soil surface represented