Identification of U p 47 in three thermophilic archaea, one mesophilic archaeon, and one hyperthermophilic bacterium

Analysis of the profile of the tRNA modifications in several allowed us to observe a novel modified uridine in the V-loop of several tRNAs from two species: and Recently, Ohira and colleagues characterized 2'-phosphouridine (U ) at position 47 in tRNAs of thermophilic , as well as in several other archaea and thermophilic bacteria. From the presence of the gene corresponding to the RNA kinase responsible for U 47 formation, they also concluded that U 47 should be present in tRNAs of other thermophilic Reanalysis of our earlier data confirms that the unidentified residue in tRNAs of both and is indeed 2'-phosphouridine followed by m C48. Moreover, we find this modification in several tRNAs of other and of the hyperthermophilic bacterium .