Evaluation of Larger Test Portion Sizes for Escherichia coli Shiga Toxin Producer (STEC) on the Detection by Immunomagnetic Separation and Real-Time PCR in Meat and Vegetables
The aims of this study were (i) to evaluate the performance of the Assurance ® GDS method combining immunomagnetic separation and real-time PCR for STEC detection in pooled samples (up to 375g) of vegetables and meat, and (ii) to compare its performances to that of the reference method ISO/TS-13136:...
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Veröffentlicht in: | Food analytical methods 2023-07, Vol.16 (7), p.1271-1282 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | The aims of this study were (i) to evaluate the performance of the Assurance
®
GDS method combining immunomagnetic separation and real-time PCR for STEC detection in pooled samples (up to 375g) of vegetables and meat, and (ii) to compare its performances to that of the reference method ISO/TS-13136:2012 (25 g sample size) in artificially contaminated samples. The alternative Assurance
®
GDS method includes enrichment in proprietary broth at 41.5°C for 10 h followed by primary screening of TOP 7 STEC containing
eae
,
stx
genes and O157:H7 markers using MPX-Top 7 (IMS+PCR), secondary screening for serogroup identification using MPX-ID/EHEC-ID (IMS+PCR). And finally, cultural confirmation from same IMS beads on two selective agars is set up. For reference method, the enrichment was performed in BPW at 41.5°C for 18 h. A total of 120 samples of meat and vegetables, including 20 uninoculated and 100 samples spiked with stressed cells ( |
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ISSN: | 1936-9751 1936-976X |
DOI: | 10.1007/s12161-023-02505-5 |