Arabidopsis seeds altered in the circadian clock protein TOC1 are characterized by higher level of linolenic acid

The circadian clock plays a critical role in regulating plant physiology and metabolism. However, the way in which the clock impacts the regulation of lipid biosynthesis in seeds is partially understood. In the present study, we characterized the seed fatty acid (FA) and glycerolipid (GL) compositions of pseudo-response regulator mutants. Among these mutants, toc1 (timing of cab expression 1) exhibited the most significant differences compared to control plants. These included an increase in total FA content, characterized by elevated levels of linolenic acid (18:3) along with a reduction in linoleic acid (18:2). Furthermore, our findings revealed that toc1 developing seeds showed increased expression of genes related to FA metabolism. Our results show a connection between TOC1 and lipid metabolism in Arabidopsis seeds. •TOC1 influences FA synthesis in Arabidopsis seeds.•Increased levels of linolenic acid in toc1 seeds.•Higher expression of lipid-related genes in developing toc1 seeds.•TOC1 connects the clock and FA metabolism.