ASSESSMENT OF MITOCHONDRIAL REDOX ACTIVITY DURING CRYOPRESERVATION PROCESS OF INDIAN RED JUNGLE FOWL (GALLUS GALLUS MURGHI) SPERM THROUGH MTT REDUCTION ASSAY

ASSESSMENT OF MITOCHONDRIAL REDOX ACTIVITY DURING CRYOPRESERVATION PROCESS OF INDIAN RED JUNGLE FOWL (GALLUS GALLUS MURGHI) SPERM THROUGH MTT REDUCTION ASSAY

The experiment was conducted to evaluate the metabolic activity through MTT (3[4,5-dimethylthiazol-2-y1]-2,5-diphenyltetrazolium bromide) reduction assay of Indian red jungle fowl sperm during cryopreservation process. In addition, MTT test was compared with sperm motility and viability data. Sperm...

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Veröffentlicht in:Journal of animal and plant sciences 2023-02, Vol.33 (1), p.229-234
Hauptverfasser: Rakha, B.A., Ansari, M.S., Akhter, S, Akhter, A, Blesbois, E, Mareno, J.S.
Format: Artikel
Sprache:eng
Schlagworte:
Life Sciences
Reproductive Biology
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Zusammenfassung:The experiment was conducted to evaluate the metabolic activity through MTT (3[4,5-dimethylthiazol-2-y1]-2,5-diphenyltetrazolium bromide) reduction assay of Indian red jungle fowl sperm during cryopreservation process. In addition, MTT test was compared with sperm motility and viability data. Sperm analysis was made at pre-dilution, post-dilution, post-cooling, post-equilibration and post freeze-thawing stages. Pearson’s correlation coefficient showed significant (P
ISSN:1018-7081
2309-8694
DOI:10.36899/JAPS.2023.1.0613