Intraperitoneal insufflation of carbon dioxide rescues intestinal damage in an experimental murin model of colitis

Objectives: Necrotizing enterocolitis (NEC) is a severe neonatal surgical condition, associated with a prolonged pro-inflammatory state, leading to high mortality and morbidity rates. Carbon dioxide (CO2) insufflation during laparoscopy may have an anti-inflammatory effect. We aimed to evaluate the effects of CO2-insufflation on experimental colitis.Methods: Acute colitis was induced in 6-week-old Balb/c mice by the administration of 2%-dextran sulfate-sodium (DSS) during 7 days (n = 45). On Day 4, two groups received intraperitoneal insufflation (duration: 30 mn, pressure: 5 mmHg) of CO2 (“DSS+CO2”) or air (“DSS+air”). A group received no insufflation (“DSS”). Groups were compared for clinical severity using the disease activity index (DAI—body weight loss, stool consistency, and bleeding), histological severity (histopathological activity index, colon length, and ulcerations), colonic mucosecretion, and inflammation.Results: DAI was significantly decreased in DSS+CO2 group, compared to DSS (p