Vitislactone, a non-canonical strigolactone exudated by grapevine rootstocks in response to nitrogen starvation

Strigolactones are compounds produced by plant roots in response to nutrient deficiency, acting both as local and systemic signals to control development and nutrition. Strigolactones are exuded in the rhizosphere to positively influence interactions with beneficial microbes. LC-MS/MS analysis shows that two genetically distinct grapevine rootstocks exudate one or two non-canonical strigolactones when subjected to low nitrogen conditions. Gene expression profiles and orobanche seed germination assays confirm that the biosynthesis and exudation of non-canonical compounds is the preferred pathway. The first compound, corresponding to heliolactone or 6-epi-heliolactone, is only exuded by the rootstock showing lower shoot branching and a higher level of mycorrhization with arbuscular mycorrhizal fungi. The structure of the second compound exuded by both rootstocks was identified by NMR and LC-MS/MS analysis. It is a non-canonical strigolactone, which has never been identified in another species. This first identification of a natural compound with the potential to stimulate beneficial root-microbe interactions in grapevines opens new perspectives in viticulture. [Display omitted] •Non-canonical Strigolactone compounds were identified in root exudates of grapevine rootstocks•Nitrogen deprivation increases exudation strigolactone compounds in grapevine rootstocks.•Vitislactone showed an original non-canonical feature.•Heliolactone-like compound was found only in exudates of Vitis riparia Gloire de Montpellier rootstock.