Development of SNP markers for Ayous (Triplochiton scleroxylon K. Schum) an economically important tree species from tropical West and Central Africa

Development of SNP markers for Ayous (Triplochiton scleroxylon K. Schum) an economically important tree species from tropical West and Central Africa

182 SNP markers were developed for Ayous (Triplochiton scleroxylon K. Schum) by incorporating information from two next generation sequencing approaches (RADseq Floragenex and AFLPseq IonTorrent PGM) into a single genotyping panel for MassARRAY® iPLEX™. This set of markers was successfully used t...

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Veröffentlicht in:Conservation genetics resources 2016-06, Vol.8 (2), p.129-139
Hauptverfasser: Jardine, D. I, C. Blanc-Jolivet, R. R. M. Dixon, E. E. Dormontt, B. Dunker, J. Gerlach, B. Kersten, K.-J. van Dijk, B. Degen, A. J. Lowe
Format: Artikel
Sprache:eng
Schlagworte:
Animal Genetics and Genomics
Biodiversity
Biomedical and Life Sciences
Conservation Biology/Ecology
Ecology
Evolutionary Biology
Genetics
Genotypes
Genotyping
Heterozygosity
high-throughput nucleotide sequencing
Life Sciences
loci
Next-generation sequencing
Plant Genetics and Genomics
Populations and Evolution
Single-nucleotide polymorphism
Technical Note
trees
Triplochiton scleroxylon
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Zusammenfassung:182 SNP markers were developed for Ayous (Triplochiton scleroxylon K. Schum) by incorporating information from two next generation sequencing approaches (RADseq Floragenex and AFLPseq IonTorrent PGM) into a single genotyping panel for MassARRAY® iPLEX™. This set of markers was successfully used to genotype 753 individuals from 43 populations in five Tropical West and Central African Countries. These loci have an expected heterozygosity range of 0.007–0.501 and F ST from 0 to 0.306.
ISSN:1877-7252
1877-7260
DOI:10.1007/s12686-016-0529-8