The Role of BEAMing and Digital PCR for Multiplexed Analysis in Molecular Oncology in the Era of Next-Generation Sequencing

BEAMing polymerase chain reaction (PCR) and digital PCR (dPCR) are used for robust and accurate quantification of nucleic acids. These methods are particularly well suited for the identification of very small fractions (

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Bibliographische Detailangaben
Veröffentlicht in:	Molecular diagnosis & therapy 2017-12, Vol.21 (6), p.587-600
Hauptverfasser:	Denis, Jérôme Alexandre, Guillerm, Erell, Coulet, Florence, Larsen, Annette K., Lacorte, Jean-Marc
Format:	Artikel
Sprache:	eng
Schlagworte:	Automation Bacteriology Biomedical and Life Sciences Biomedicine Biopsy Breast cancer Cancer Cancer Research Colorectal cancer Deoxyribonucleic acid DNA DNA methylation DNA Mutational Analysis - methods Genes Genomics High-Throughput Nucleotide Sequencing - methods Human Genetics Humans Identification methods Laboratory Medicine Leading Article Life Sciences Metastasis Molecular Medicine Multiplex Polymerase Chain Reaction - methods Multiplexing Mutation Neoplasms - genetics Nucleic acids Oncology Pharmacotherapy Polymerase chain reaction Polymerase Chain Reaction - methods Trends Virology
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Beschreibung
Zusammenfassung:	BEAMing polymerase chain reaction (PCR) and digital PCR (dPCR) are used for robust and accurate quantification of nucleic acids. These methods are particularly well suited for the identification of very small fractions (
ISSN:	1177-1062 1179-2000
DOI:	10.1007/s40291-017-0287-7