Biosensing Extracellular Vesicle Subpopulations in Neurodegenerative Disease Conditions

Extracellular vesicles (EVs) are secreted nanoparticles that are involved in intercellular communication and that modulate a wide range of biological processes in normal and disease conditions. However, EVs are highly heterogeneous in terms of origin in the cell, size, and density. As a result, comp...

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Veröffentlicht in:ACS sensors 2022-06, Vol.7 (6), p.1657-1665
Hauptverfasser: Mazouzi, Yacine, Sallem, Fadoua, Farina, Francesca, Loiseau, Alexis, Tartaglia, Natayme Rocha, Fontaine, Morgane, Parikh, Atul, Salmain, Michèle, Neri, Christian, Boujday, Souhir
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Sprache:eng
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Zusammenfassung:Extracellular vesicles (EVs) are secreted nanoparticles that are involved in intercellular communication and that modulate a wide range of biological processes in normal and disease conditions. However, EVs are highly heterogeneous in terms of origin in the cell, size, and density. As a result, complex protocols are required to identify and characterize specific EV subpopulations, limiting biomedical applications, notably in diagnostics. Here, we show that combining quartz crystal microbalance with dissipation (QCM-D) and nanoplasmonic sensing (NPS) provides a facile method to track the viscoelastic properties of small EVs. We applied this multisensing strategy to analyze small EVs isolated by differential ultracentrifugation from knock-in mouse striatal cells expressing either a mutated allele or wild-type allele of huntingtin (Htt), the Huntington’s disease gene. Our results validate the sensing strategy coupling QCM-D and NPS and suggest that the mass and viscoelastic dissipation of EVs can serve as potent biomarkers for sensing the intercellular changes associated with the neurodegenerative condition.
ISSN:2379-3694
2379-3694
DOI:10.1021/acssensors.1c02658