Studying Cell Division Plane Positioning in Early-Stage Embryos
Unraveling the mechanisms that govern division plane orientation is a major challenge to understand plant development. In this respect, the Arabidopsis early embryo is a model system of choice since embryogenesis is relatively simple and cell division planes orientation is highly predictable. Here w...
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| Veröffentlicht in: | Methods in molecular biology (Clifton, N.J.) N.J.), 2022, Vol.2382, p.141-154 |
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| container_title | Methods in molecular biology (Clifton, N.J.) |
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| creator | Belcram, Katia Palauqui, Jean-Christophe Pastuglia, Martine |
| description | Unraveling the mechanisms that govern division plane orientation is a major challenge to understand plant development. In this respect, the Arabidopsis early embryo is a model system of choice since embryogenesis is relatively simple and cell division planes orientation is highly predictable. Here we present an integrated set of protocols to study 3D cell division patterns in early-stage Arabidopsis embryos that combine both cellular and sub-cellular localization of selected protein markers with spatial organization of cells, cytoskeleton, and nuclei. |
| doi_str_mv | 10.1007/978-1-0716-1744-1_9 |
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| source | MEDLINE; Springer Books |
| subjects | Arabidopsis Arabidopsis Proteins Cell Division Cell wall Embryo Fluorescent marker Immunolocalization Life Sciences Microtubules Plant Development Whole-mount |
| title | Studying Cell Division Plane Positioning in Early-Stage Embryos |
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