Studying Cell Division Plane Positioning in Early-Stage Embryos

Studying Cell Division Plane Positioning in Early-Stage Embryos

Unraveling the mechanisms that govern division plane orientation is a major challenge to understand plant development. In this respect, the Arabidopsis early embryo is a model system of choice since embryogenesis is relatively simple and cell division planes orientation is highly predictable. Here w...

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Veröffentlicht in:Methods in molecular biology (Clifton, N.J.) N.J.), 2022, Vol.2382, p.141-154
Hauptverfasser: Belcram, Katia, Palauqui, Jean-Christophe, Pastuglia, Martine
Format: Artikel
Sprache:eng
Schlagworte:
Arabidopsis
Arabidopsis Proteins
Cell Division
Cell wall
Embryo
Fluorescent marker
Immunolocalization
Life Sciences
Microtubules
Plant Development
Whole-mount
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Zusammenfassung:Unraveling the mechanisms that govern division plane orientation is a major challenge to understand plant development. In this respect, the Arabidopsis early embryo is a model system of choice since embryogenesis is relatively simple and cell division planes orientation is highly predictable. Here we present an integrated set of protocols to study 3D cell division patterns in early-stage Arabidopsis embryos that combine both cellular and sub-cellular localization of selected protein markers with spatial organization of cells, cytoskeleton, and nuclei.
ISSN:1064-3745
1940-6029
DOI:10.1007/978-1-0716-1744-1_9