SAT0047 Local Cryotherapy Down-Regulates Local and Systemic IL-6 and IL-17 Pathways in Adjuvant-Induced Arthritis

BackgroundCryotherapy is widely and empirically used in an adjuvant and symptomatic setting in inflammatory rheumatic diseases, with a low level of evidence [1].ObjectivesThe aim of this work was to evaluate local and systemic anti-inflammatory effects of local cryotherapy (LC) in adjuvant-induced arthritis (AIA), comparing 2 techniques (ice and cold gas spray). We considered clinical (arthritic score and ankle diameter) and biological effects on IL-6, IL-17A, IL-1β, TNF-α local and systemic levels.MethodsArthritis was induced by a single Mycobacterium butyricum injection in male Lewis rat tails (day 0). At the onset of arthritis (day 11), rats were treated either by 30 minute-ice applications on hind paws (in cages lined with ice pops – n=10) or by 2 minute-cold gas pulverizations on both hind paws (n=9) twice a day for 14 consecutive days. Ten non-treated AIA rats were used as controls. At day 24 (the day after the last cold application), hind paws were grinded in order to measure cytokine gene expression levels by Q-RT-PCR. Plasmatic levels of the same cytokines were also measured in plasma by cytometry (Multiplex Magpix® ebioscience). IL-6 plasma levels were measured by ELISA (rat IL6 platinum ELISA, BMS625®, ebioscience).ResultsIce application significantly reduced the mean arthritic score and ankle diameter from day 6 to day 14 (p