Versatile and Affordable Approach for Tracking the Oxidative Stress Caused by the Free Radicals: the Chemical Perception

Fascinating oscillations of color, kinetics of pH, cyclic voltammetry (CV) and UV‐vis spectra, ensued from supplementing of VO(acac)2‐acetonitrile solutions with micro‐amounts of H2O2, were greatly affected by the small additives of glyoxal and oxalic acid. Such effect was consisted in sufficient decreasing the sensitivity of samples by means of it pH, CV and UV‐vis response onto H2O2 additives. The revealed peculiarities substantiate the responsibility of free radicals (particularly .OH generated in the course of H2O2 homolysis) for the discussed parameters alteration. In view of .OH are identified as most active and destructive (especially, in respect to the cellular constituents) agents can be originated in vivo, it overproduction is responsible for developing of many related severe diseases. In practical aspect the undertaken research may be recommended for the oxidative stress evaluation and tracking. We report the efficient and economic approach to the express method of oxidative stress tracking. Hydroxyl radicals, originated from even traces of H2O2, are responsible for sharp decrease of pH as well as UV‐vis and CV spectra changing. The revealed feature can be used to quantify the content of H2O2 in analyzed patterns and reckon the impact of free radicals as well.