Cell-free biosensors for biomedical applications

Recent years have witnessed a rapid increase of the use of cell-free systems for biosensor engineering. Building on the past decade of synthetic biology research mostly performed in living cells, researchers have developed a variety of cell-free applications capable of detecting antibiotics, clinica...

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Veröffentlicht in:Current opinion in biomedical engineering 2020-03, Vol.13, p.9-15
Hauptverfasser: Voyvodic, Peter L., Bonnet, Jerome
Format: Artikel
Sprache:eng
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Zusammenfassung:Recent years have witnessed a rapid increase of the use of cell-free systems for biosensor engineering. Building on the past decade of synthetic biology research mostly performed in living cells, researchers have developed a variety of cell-free applications capable of detecting antibiotics, clinical biomarkers, or nucleic acid sequences from viral and bacterial pathogens. Cell-free systems allow for simple fine-tuning of circuit component stoichiometry and provide a nonliving alternative to whole-cell biosensors that could expedite their path through regulatory approval. In addition, many of these systems have been shown to exhibit increased stability upon lyophilization, potentially enabling point-of-care applications and use in low-resource settings where continuous cold-chain maintenance may be difficult. Here, we discuss the latest advances in cell-free biosensor research and highlight some key challenges to address for the field to bring its full potential.
ISSN:2468-4511
2468-4511
DOI:10.1016/j.cobme.2019.08.005