A novel NADPH‐dependent aldehyde reductase gene from Vigna radiata confers resistance to the grapevine fungal toxin eutypine
Summary Eutypine, 4‐hydroxy‐3‐(3‐methyl‐3‐butene‐1‐ynyl) benzyl aldehyde, is a toxin produced by Eutypa lata, the causal agent of eutypa dieback of grapevines. It has previously been demonstrated that tolerance of some cultivars to this disease was correlated with their capacity to convert eutypine...
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Veröffentlicht in: | The Plant journal : for cell and molecular biology 1998-11, Vol.16 (3), p.335-343 |
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Sprache: | eng |
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Zusammenfassung: | Summary
Eutypine, 4‐hydroxy‐3‐(3‐methyl‐3‐butene‐1‐ynyl) benzyl aldehyde, is a toxin produced by
Eutypa lata,
the causal agent of eutypa dieback of grapevines. It has previously been demonstrated that tolerance of some cultivars to this disease was correlated with their capacity to convert eutypine to the corresponding alcohol, eutypinol, which lacks phytotoxicity. We have thus purified to homogeneity a protein from
Vigna radiata
that exhibited eutypine‐reducing activity and have isolated the corresponding cDNA. This encodes an NADPH‐dependent reductase of 36 kDa that we have named
Vigna radiata
eutypine‐reducing enzyme (VR‐ERE), based on the capacity of a recombinant form of the protein to reduce eutypine into eutypinol. The strongest homologies (86.8%) of VR‐ERE at the amino acid level were found with CPRD14, a drought‐inducible gene of unknown function, isolated from
Vigna unguiculata
and with an aromatic alcohol dehydrogenase (71.7%) from
Eucalyptus gunnii
. Biochemical characterization of VR‐ERE revealed that a variety of compounds containing an aldehyde group can act as substrates. However, the highest affinity was observed with 3‐substituted benzaldehydes. Expression of a
VR‐ERE
transgene in
Vitis vinifera
cells cultured
in vitro
conferred resistance to the toxin. This discovery opens up new biotechnological approaches for the generation of grapevines resistant to eutypa dieback. |
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ISSN: | 0960-7412 1365-313X |
DOI: | 10.1046/j.1365-313x.1998.00303.x |