Micropropagation of Carob (Ceratonia silique L.) through Adventitious Buds of Immature Embryonic Cotyledons

Micropropagation of Carob (Ceratonia silique L.) through Adventitious Buds of Immature Embryonic Cotyledons

Adventitious budding from embryonic cotyledons of immature seeds of carob was obtained. The combination of BAP (4.44 μM) and NAA (1.5 μM) furthered the neoformation of adventitious buds. These latter were multiplied on MS medium added with BAP (2.22 μM). Stems and leaves growing were improved by add...

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Veröffentlicht in:American journal of plant sciences 2017, Vol.8 (9), p.2180-2195
Hauptverfasser: Bouzdoudi, Brahim El, Saïdi, Rabah, El Ansari, Zineb Nejjar, El Kbiach, Mohammed L’bachir, Martin, Patrick, Badoc, Alain, Lamarti, Ahmed
Format: Artikel
Sprache:eng
Schlagworte:
Botanics
Life Sciences
Vegetal Biology
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Zusammenfassung:Adventitious budding from embryonic cotyledons of immature seeds of carob was obtained. The combination of BAP (4.44 μM) and NAA (1.5 μM) furthered the neoformation of adventitious buds. These latter were multiplied on MS medium added with BAP (2.22 μM). Stems and leaves growing were improved by adding 2.02 μM GA3. Elongation was favored by 0.5 μM NAA. 70% of rooting was obtained with 10 μM IBA.
ISSN:2158-2742
2158-2750
DOI:10.4236/ajps.2017.89146