Draft genome sequence of Promicromonospora panici sp. nov., a novel ionizing-radiation-resistant actinobacterium isolated from roots of the desert plant Panicum turgidum

A novel strain of the genus Promicromonospora , designated PT9 T , was recovered from irradiated roots of the xerophyte Panicum turgidum collected from the Ksar Ghilane oasis in southern Tunisia. Strain PT9 T is aerobic, non-spore-forming, Gram- positive actinomycete that produces branched hyphae and forms white to yellowish-white colonies. Chemotaxonomic features, including fatty acids, whole cell sugars and polar lipid profiles, support the assignment of PT9 T to the genus Promicromonospora . The genomic relatedness indexes based on DNA-DNA hybridization and average nucleotide identity values revealed a significant genomic divergence between strain PT9 T and all sequenced type strains of the taxon. Phylogenomic analysis showed that isolate PT9 T was most closely related to Promicromonospora soli CGMCC 4.7398 T . Phenotypic and phylogenomic analyses suggest that isolate PT9 T represents a novel species of the genus Promicromonospora , for which the name Promicromonospora panici sp. nov. is proposed. The type strain is PT9 T (LMG 31103 T = DSM 108613 T ).The isolate PT9 T is an ionizing-radiation-resistant actinobacterium ( D 10 value = 2.6 kGy), with resistance to desiccation and hydrogen peroxide. The complete genome sequence of PT9 T consists of 6,582,650 bps with 71.2% G+C content and 6291 protein-coding sequences. This genome will help to decipher the microbial genetic bases for ionizing-radiation resistance mechanisms including the response to oxidative stress.