105  Sexual dimorphism in equine D8 blastocysts

In mammals, male embryos grow faster. Equine embryo size is highly variable for same developmental stage, with no reported relation to embryo sex nor gene expression. Our objective was to evaluate gene expression sexual dimorphism on equine D8 blastocysts. Non-nursing, Saddlebred mares located on 2 experimental farms were inseminated with the semen of one unique stallion per farm. At 8 d post ovulation, 17 and 11 embryos from each farm, each from a different dam, were recovered by uterine flushing and bissected to obtain samples of pure (TE) or inner cell mass enriched (TE-ICM) trophoblast. RNA expression was analyzed separately in the TE-ICM and the TE of 16 female and 12 male embryos using paired end, non-oriented RNA sequencing (Illumina, NextSeq500). To discriminate gene expression in the ICM from that in the TE, deconvolution (DeMixT R package) was used on the TE-ICM data. Differential expression was analyzed (DESeq2) with farm and embryo size as cofactors using a false discovery rate (FDR) < 0.05 cutoff. Gene Set Enrichment Analysis was performed using the KEGG and GO BP databases. Out of the 13,786 and 13,210 genes expressed in ICM and TE, respectively, only 184 (51.1% on X chromosome) in ICM and 415 (39.0% on X chromosome) in TE were differentially expressed, with 111 genes in common. Overall, 145 in ICM and 312 in TE genes were significantly upregulated in female. As reported in bovine embryos, glucose-6-phosphate dehydrogenase (G6PD) was upregulated in female (log2 Fold Change = 0.96 in ICM and 0.83 in TE; both FDR