Probing Bacterial Adhesion at the Single-Molecule and Single-Cell Levels by AFM-Based Force Spectroscopy

Probing Bacterial Adhesion at the Single-Molecule and Single-Cell Levels by AFM-Based Force Spectroscopy

Functionalization of AFM probes with biomolecules or microorganisms allows for a better understanding of the interaction mechanisms driving microbial adhesion. Here we describe the most commonly used protocols to graft molecules and bacteria to AFM cantilevers. The bioprobes obtained that way enable...

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Veröffentlicht in:Methods in molecular biology (Clifton, N.J.) N.J.), 2018-01, Vol.1814, p.403-414
Hauptverfasser: El-Kirat-Chatel, Sofiane, Beaussart, Audrey
Format: Artikel
Sprache:eng
Schlagworte:
Atomic force microscopy
Bacteriology
Functionalization
Interactions
Life Sciences
Microbiology and Parasitology
Single-cell
Single-molecule
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Zusammenfassung:Functionalization of AFM probes with biomolecules or microorganisms allows for a better understanding of the interaction mechanisms driving microbial adhesion. Here we describe the most commonly used protocols to graft molecules and bacteria to AFM cantilevers. The bioprobes obtained that way enable to measure forces down to the single-cell and single-molecule levels.
ISSN:1064-3745
1940-6029
DOI:10.1007/978-1-4939-8591-3_24