Protein-RNA crosslinking in the subunits of the cytoplasmic ribosome of Tetrahymena thermophila

Use of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide to introduce RNA-protein crosslinks in the 40S and 60S subunits of the cytoplasmic ribosome of Tetrahymena thermophila is described, and proteins linked covalently to 17S and 26S ribosomal RNAs are identified. RNA-protein crosslinking is accompan...

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Veröffentlicht in:	Biochimie 1989-05, Vol.71 (5), p.667-679
Hauptverfasser:	Petridou, Barbara, Guerin, Marie-France, Hayes, Françoise
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences carbodiimide Cell structures and functions Centrifugation, Density Gradient Cross-Linking Reagents crosslinking Cytoplasm - metabolism Electrophoresis, Gel, Two-Dimensional Electrophoresis, Polyacrylamide Gel Ethyldimethylaminopropyl Carbodiimide Fundamental and applied biological sciences. Psychology Life Sciences Molecular and cellular biology Peptide Mapping Ribosomal Proteins - metabolism Ribosome Ribosomes - drug effects Ribosomes - metabolism RNA - metabolism Tetrahymena Tetrahymena - drug effects Tetrahymena - metabolism
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Zusammenfassung:	Use of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide to introduce RNA-protein crosslinks in the 40S and 60S subunits of the cytoplasmic ribosome of Tetrahymena thermophila is described, and proteins linked covalently to 17S and 26S ribosomal RNAs are identified. RNA-protein crosslinking is accompanied by extensive dimerization and aggregation of ribosomal subunits probably due to formation of interparticle protein-protein crosslinks.
ISSN:	0300-9084 1638-6183
DOI:	10.1016/0300-9084(89)90161-2