Paternal age: Negative impact on sperm genome decays and IVF outcomes after 40 years
This study assessed sperm quality declining on relation to paternal age and its impact on in vitro fertilization (IVF) outcomes in order to estimate the APA (Advanced Paternal Age) cutoff. For this, 83 couples undergoing IVF treatment for male factor infertility were enrolled. The women age was ≤39...
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| Veröffentlicht in: | Molecular reproduction and development 2018-03, Vol.85 (3), p.271-280 |
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| creator | Kaarouch, Ismail Bouamoud, Nouzha Madkour, Aicha Louanjli, Noureddine Saadani, Brahim Assou, Said Aboulmaouahib, Smahane Amzazi, Saaid Copin, Henri Benkhalifa, Moncef Sefrioui, Omar |
| description | This study assessed sperm quality declining on relation to paternal age and its impact on in vitro fertilization (IVF) outcomes in order to estimate the APA (Advanced Paternal Age) cutoff. For this, 83 couples undergoing IVF treatment for male factor infertility were enrolled. The women age was ≤39 years, whereas the men were divided in two groups: APA (n = 41; age ≥ 40 years) and young (Y) (n = 42; age |
| doi_str_mv | 10.1002/mrd.22963 |
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For this, 83 couples undergoing IVF treatment for male factor infertility were enrolled. The women age was ≤39 years, whereas the men were divided in two groups: APA (n = 41; age ≥ 40 years) and young (Y) (n = 42; age < 40 years). Conventional semen parameters (volume, concentration, motility, vitality, and morphology) were analyzed in the collected sperm samples. Furthermore, sperm genome decays (SGD) was assessed by TUNEL assay (DNA fragmentation), aniline blue staining (chromatin decondensation), and fluorescent in situ hybridization (aneuploidy). No significant difference was found concerning the conventional semen parameters between APA and Y groups. Conversely, SGD analysis showed increased DNA fragmentation; chromatin decondensation and sperm aneuploidy rates in the APA group (respectively, 41%, 43%, and 14% vs. 25%, 23%, and 4% in Y group). IVF outcomes also were affected by paternal age as indicated by the rates of cancelled embryo transfers, clinical pregnancy and miscarriage in the two groups APA and Y (29%, 17%, and 60% vs. 10%, 32%, and 42%). Finally, statistical analysis of the results suggests that the age of 40 should be considered as the APA cutoff during ART attempts.</description><identifier>ISSN: 1040-452X</identifier><identifier>EISSN: 1098-2795</identifier><identifier>DOI: 10.1002/mrd.22963</identifier><identifier>PMID: 29392876</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>advanced paternal age ; Age ; Aneuploidy ; Aniline ; Chromatin ; DNA fragmentation ; Fluorescence in situ hybridization ; Genomes ; In vitro fertilization ; in vitro fertilization outcomes ; Infertility ; Life Sciences ; Pregnancy ; Semen ; Sperm ; sperm genome decays ; sperm quality ; Statistical analysis</subject><ispartof>Molecular reproduction and development, 2018-03, Vol.85 (3), p.271-280</ispartof><rights>2018 Wiley Periodicals, Inc.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4533-23fc587f374605cd81019c45907cd8703ff07554c77442b1113cff56a7adaf53</citedby><cites>FETCH-LOGICAL-c4533-23fc587f374605cd81019c45907cd8703ff07554c77442b1113cff56a7adaf53</cites><orcidid>0000-0002-3382-989X ; 0000-0001-6448-7995</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fmrd.22963$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fmrd.22963$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,776,780,881,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29392876$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-03158098$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Kaarouch, Ismail</creatorcontrib><creatorcontrib>Bouamoud, Nouzha</creatorcontrib><creatorcontrib>Madkour, Aicha</creatorcontrib><creatorcontrib>Louanjli, Noureddine</creatorcontrib><creatorcontrib>Saadani, Brahim</creatorcontrib><creatorcontrib>Assou, Said</creatorcontrib><creatorcontrib>Aboulmaouahib, Smahane</creatorcontrib><creatorcontrib>Amzazi, Saaid</creatorcontrib><creatorcontrib>Copin, Henri</creatorcontrib><creatorcontrib>Benkhalifa, Moncef</creatorcontrib><creatorcontrib>Sefrioui, Omar</creatorcontrib><title>Paternal age: Negative impact on sperm genome decays and IVF outcomes after 40 years</title><title>Molecular reproduction and development</title><addtitle>Mol Reprod Dev</addtitle><description>This study assessed sperm quality declining on relation to paternal age and its impact on in vitro fertilization (IVF) outcomes in order to estimate the APA (Advanced Paternal Age) cutoff. For this, 83 couples undergoing IVF treatment for male factor infertility were enrolled. The women age was ≤39 years, whereas the men were divided in two groups: APA (n = 41; age ≥ 40 years) and young (Y) (n = 42; age < 40 years). Conventional semen parameters (volume, concentration, motility, vitality, and morphology) were analyzed in the collected sperm samples. Furthermore, sperm genome decays (SGD) was assessed by TUNEL assay (DNA fragmentation), aniline blue staining (chromatin decondensation), and fluorescent in situ hybridization (aneuploidy). No significant difference was found concerning the conventional semen parameters between APA and Y groups. Conversely, SGD analysis showed increased DNA fragmentation; chromatin decondensation and sperm aneuploidy rates in the APA group (respectively, 41%, 43%, and 14% vs. 25%, 23%, and 4% in Y group). IVF outcomes also were affected by paternal age as indicated by the rates of cancelled embryo transfers, clinical pregnancy and miscarriage in the two groups APA and Y (29%, 17%, and 60% vs. 10%, 32%, and 42%). Finally, statistical analysis of the results suggests that the age of 40 should be considered as the APA cutoff during ART attempts.</description><subject>advanced paternal age</subject><subject>Age</subject><subject>Aneuploidy</subject><subject>Aniline</subject><subject>Chromatin</subject><subject>DNA fragmentation</subject><subject>Fluorescence in situ hybridization</subject><subject>Genomes</subject><subject>In vitro fertilization</subject><subject>in vitro fertilization outcomes</subject><subject>Infertility</subject><subject>Life Sciences</subject><subject>Pregnancy</subject><subject>Semen</subject><subject>Sperm</subject><subject>sperm genome decays</subject><subject>sperm quality</subject><subject>Statistical analysis</subject><issn>1040-452X</issn><issn>1098-2795</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp1kUtLAzEUhYMovhf-AQm40cXUm2QymbgrvirUB1LEXYiZpI7MoyYzSv-9qfUBgqskJx_nXs5BaI_AgADQ49oXA0plxlbQJgGZJ1RIvrq4p5CknD5uoK0QXgBAyhzW0QaVTNJcZJtocqc76xtdYT21J_jGTnVXvllc1jNtOtw2OMysr_HUNm1tcWGNngesmwJfPVzgtu9MlKPgogtOAc-t9mEHrTldBbv7dW6jycX55HSUjG8vr06H48SknLGEMmd4LhwTaQbcFDkBIuOXBBEfAphzIDhPjRBpSp8IIcw4xzMtdKEdZ9voaGn7rCs182Wt_Vy1ulSj4VgtNGCE5zGPNxLZwyU78-1rb0On6jIYW1W6sW0fFJExEplRISJ68Ad9aftFREFRIIzGBTP2O9z4NgRv3c8GBNSiFRVbUZ-tRHb_y7F_qm3xQ37XEIHjJfBeVnb-v5O6vj9bWn4AfteStg</recordid><startdate>201803</startdate><enddate>201803</enddate><creator>Kaarouch, Ismail</creator><creator>Bouamoud, Nouzha</creator><creator>Madkour, Aicha</creator><creator>Louanjli, Noureddine</creator><creator>Saadani, Brahim</creator><creator>Assou, Said</creator><creator>Aboulmaouahib, Smahane</creator><creator>Amzazi, Saaid</creator><creator>Copin, Henri</creator><creator>Benkhalifa, Moncef</creator><creator>Sefrioui, Omar</creator><general>Wiley Subscription Services, Inc</general><general>Wiley</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-3382-989X</orcidid><orcidid>https://orcid.org/0000-0001-6448-7995</orcidid></search><sort><creationdate>201803</creationdate><title>Paternal age: Negative impact on sperm genome decays and IVF outcomes after 40 years</title><author>Kaarouch, Ismail ; Bouamoud, Nouzha ; Madkour, Aicha ; Louanjli, Noureddine ; Saadani, Brahim ; Assou, Said ; Aboulmaouahib, Smahane ; Amzazi, Saaid ; Copin, Henri ; Benkhalifa, Moncef ; Sefrioui, Omar</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4533-23fc587f374605cd81019c45907cd8703ff07554c77442b1113cff56a7adaf53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>advanced paternal age</topic><topic>Age</topic><topic>Aneuploidy</topic><topic>Aniline</topic><topic>Chromatin</topic><topic>DNA fragmentation</topic><topic>Fluorescence in situ hybridization</topic><topic>Genomes</topic><topic>In vitro fertilization</topic><topic>in vitro fertilization outcomes</topic><topic>Infertility</topic><topic>Life Sciences</topic><topic>Pregnancy</topic><topic>Semen</topic><topic>Sperm</topic><topic>sperm genome decays</topic><topic>sperm quality</topic><topic>Statistical analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kaarouch, Ismail</creatorcontrib><creatorcontrib>Bouamoud, Nouzha</creatorcontrib><creatorcontrib>Madkour, Aicha</creatorcontrib><creatorcontrib>Louanjli, Noureddine</creatorcontrib><creatorcontrib>Saadani, Brahim</creatorcontrib><creatorcontrib>Assou, Said</creatorcontrib><creatorcontrib>Aboulmaouahib, Smahane</creatorcontrib><creatorcontrib>Amzazi, Saaid</creatorcontrib><creatorcontrib>Copin, Henri</creatorcontrib><creatorcontrib>Benkhalifa, Moncef</creatorcontrib><creatorcontrib>Sefrioui, Omar</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Molecular reproduction and development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kaarouch, Ismail</au><au>Bouamoud, Nouzha</au><au>Madkour, Aicha</au><au>Louanjli, Noureddine</au><au>Saadani, Brahim</au><au>Assou, Said</au><au>Aboulmaouahib, Smahane</au><au>Amzazi, Saaid</au><au>Copin, Henri</au><au>Benkhalifa, Moncef</au><au>Sefrioui, Omar</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Paternal age: Negative impact on sperm genome decays and IVF outcomes after 40 years</atitle><jtitle>Molecular reproduction and development</jtitle><addtitle>Mol Reprod Dev</addtitle><date>2018-03</date><risdate>2018</risdate><volume>85</volume><issue>3</issue><spage>271</spage><epage>280</epage><pages>271-280</pages><issn>1040-452X</issn><eissn>1098-2795</eissn><abstract>This study assessed sperm quality declining on relation to paternal age and its impact on in vitro fertilization (IVF) outcomes in order to estimate the APA (Advanced Paternal Age) cutoff. For this, 83 couples undergoing IVF treatment for male factor infertility were enrolled. The women age was ≤39 years, whereas the men were divided in two groups: APA (n = 41; age ≥ 40 years) and young (Y) (n = 42; age < 40 years). Conventional semen parameters (volume, concentration, motility, vitality, and morphology) were analyzed in the collected sperm samples. Furthermore, sperm genome decays (SGD) was assessed by TUNEL assay (DNA fragmentation), aniline blue staining (chromatin decondensation), and fluorescent in situ hybridization (aneuploidy). No significant difference was found concerning the conventional semen parameters between APA and Y groups. Conversely, SGD analysis showed increased DNA fragmentation; chromatin decondensation and sperm aneuploidy rates in the APA group (respectively, 41%, 43%, and 14% vs. 25%, 23%, and 4% in Y group). IVF outcomes also were affected by paternal age as indicated by the rates of cancelled embryo transfers, clinical pregnancy and miscarriage in the two groups APA and Y (29%, 17%, and 60% vs. 10%, 32%, and 42%). Finally, statistical analysis of the results suggests that the age of 40 should be considered as the APA cutoff during ART attempts.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>29392876</pmid><doi>10.1002/mrd.22963</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-3382-989X</orcidid><orcidid>https://orcid.org/0000-0001-6448-7995</orcidid></addata></record> |
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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | advanced paternal age Age Aneuploidy Aniline Chromatin DNA fragmentation Fluorescence in situ hybridization Genomes In vitro fertilization in vitro fertilization outcomes Infertility Life Sciences Pregnancy Semen Sperm sperm genome decays sperm quality Statistical analysis |
| title | Paternal age: Negative impact on sperm genome decays and IVF outcomes after 40 years |
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