Identifying roles of the scion and the rootstock in regulating plant development and functioning under different phosphorus supplies in grapevine

•Describe grafted grapevine responses to P supply at many levels of organization.•Show that scion can exert long-distance regulation of many root traits.•Novel work as few studies have been done on the effect of scion on rootstock.•Show importance of considering scion/rootstock interactions as a two-way process. Phosphorus is essential for plant life and plants have developed numerous strategies to maximise phosphate (Pi) acquisition and use under limited Pi supply. Here we have used reciprocal grafting to determine whether the shoot and root have different roles in regulating some of these strategies. Reciprocal grafts of two grapevine genotypes (Vitis vinifera cv. Pinot noir and V. berlandieri x V. rupestris cv. 1103 Paulsen) were produced as well as the corresponding homo-graft controls; the plants were grown in hydroponic culture and subjected to two levels of Pi supply (high (0.6 mM) or low (0.001 mM)). Biomass accumulation, root morphology, and root, stem and leaf organic acid, phosphate, nitrate and sulphate concentrations were measured. The transcript abundance of orthologues of known phosphate starvation induced (PSI) genes from Arabidopsis was also quantified. Under high Pi, the scion genotype had a large impact on plant growth, but the morphology of roots (such as root tip density) was not affected. Low Pi supply affected growth, tissue organic acid concentration, the activity of acid phosphatases released by the roots and the expression of PSI genes. Rootstock genotypes showed differences in root responses to low Pi supply, but the scion also exerted long-distance regulation of rootstock responses to low Pi, for example, modifying PSI gene expression, sulphate acquisition efficiency, the activity of acid phosphatases released by the roots and root organic acid concentrations. This work shows for the first time that the grapevine genotypes differ in their response to low Pi supply and that the scion can modify rootstock responses to the nutrient availability. This work highlights that genetic variation in shoot-borne signals can regulate root responses to Pi supply and that understanding rootstock responses to the environment must be done considering scion influence.