Alteration of the synthesis of the Clp ATP‐dependent protease affects morphological and physiological differentiation in Streptomyces

The genes of Streptomyces coelicolor A3(2) encoding catalytic subunits (ClpP) and regulatory subunits (ClpX and ClpC) of the ATP‐dependent protease family Clp were cloned, mapped and characterized. S. coelicolor contains at least two clpP genes, clpP1 and clpP2, located in tandem upstream from the c...

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Veröffentlicht in:	Molecular microbiology 1999-05, Vol.32 (3), p.505-517
Hauptverfasser:	De Crécy‐Lagard, Valérie, Servant‐Moisson, Pascale, Viala, Julie, Grandvalet, Cosette, Mazodier, Philippe
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Triphosphatases Adenosine Triphosphatases - genetics Adenosine Triphosphatases - metabolism Amino Acid Sequence Bacterial Proteins Bacterial Proteins - genetics Bacterial Proteins - metabolism Base Sequence Biochemistry, Molecular Biology Chromosome Mapping Endopeptidase Clp Gene Expression Regulation, Bacterial Genes, Bacterial Life Sciences Molecular Sequence Data Peptide Hydrolases Peptide Hydrolases - metabolism Protein Processing, Post-Translational Sequence Homology, Amino Acid Serine Endopeptidases Serine Endopeptidases - genetics Serine Endopeptidases - metabolism Species Specificity Streptomyces Streptomyces - classification Streptomyces - cytology Streptomyces - physiology Streptomyces coelicolor Transcription, Genetic
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Zusammenfassung:	The genes of Streptomyces coelicolor A3(2) encoding catalytic subunits (ClpP) and regulatory subunits (ClpX and ClpC) of the ATP‐dependent protease family Clp were cloned, mapped and characterized. S. coelicolor contains at least two clpP genes, clpP1 and clpP2, located in tandem upstream from the clpX gene, and at least two unlinked clpC genes. Disruption of the clpP1 gene in S. lividans and S. coelicolor blocks differentiation at the substrate mycelium step. Overexpression of clpP1 and clpP2 accelerates aerial mycelium formation in S. lividans, S. albus and S. coelicolor. Overproduction of ClpX accelerates actinorhodin production in S. coelicolor and activates its production in S. lividans.
ISSN:	0950-382X 1365-2958
DOI:	10.1046/j.1365-2958.1999.01364.x