Design and synthesis of cyclic and linear peptide-agarose tools for baiting interacting protein partners of GPCRs
The synthesis of affinity supports designed to identify GPCR partners is reported. A ligation strategy for the synthesis of cyclic and linear peptides covalently linked to agarose beads designed as baits to identify new interacting partners of intracellular loops of the V2 vasopressin receptor, a me...
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| Veröffentlicht in: | Bioorganic & medicinal chemistry letters 2006-02, Vol.16 (3), p.521-524 |
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| container_title | Bioorganic & medicinal chemistry letters |
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| creator | Granier, Sébastien Jean-Alphonse, Frédéric Déméné, Hélène Guillon, Gilles Pascal, Robert Mendre, Christiane |
| description | The synthesis of affinity supports designed to identify GPCR partners is reported.
A ligation strategy for the synthesis of cyclic and linear peptides covalently linked to agarose beads designed as baits to identify new interacting partners of intracellular loops of the V2 vasopressin receptor, a member of the G-protein-coupled receptor family, is reported. The peptide-resin conjugates were subsequently shown to interact specifically with a fraction of proteins present in cellular lysates. |
| doi_str_mv | 10.1016/j.bmcl.2005.10.061 |
| format | Article |
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A ligation strategy for the synthesis of cyclic and linear peptides covalently linked to agarose beads designed as baits to identify new interacting partners of intracellular loops of the V2 vasopressin receptor, a member of the G-protein-coupled receptor family, is reported. The peptide-resin conjugates were subsequently shown to interact specifically with a fraction of proteins present in cellular lysates.</description><identifier>ISSN: 0960-894X</identifier><identifier>EISSN: 1464-3405</identifier><identifier>DOI: 10.1016/j.bmcl.2005.10.061</identifier><identifier>PMID: 16289816</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Amino Acid Sequence ; Analytical, structural and metabolic biochemistry ; Biochemistry, Molecular Biology ; Biological and medical sciences ; Cell Behavior ; Cell Membrane - metabolism ; Cellular Biology ; Drug Design ; Electrophoresis, Gel, Two-Dimensional ; Fundamental and applied biological sciences. Psychology ; G-protein-coupled receptors ; General aspects, investigation methods ; Humans ; Interactions. Associations ; Intermolecular phenomena ; Intracellular loop ; Life Sciences ; Ligation ; Medical sciences ; Molecular biology ; Molecular biophysics ; Molecular Networks ; Molecular Sequence Data ; Neuropharmacology ; Neurotransmitters. Neurotransmission. Receptors ; Peptide synthesis ; Peptidergic system (neuropeptide, opioid peptide, opiates...). Adenosinergic and purinergic systems ; Peptides - chemical synthesis ; Peptides - metabolism ; Peptides, Cyclic - chemical synthesis ; Peptides, Cyclic - metabolism ; Pharmaceutical sciences ; Pharmacology ; Pharmacology. Drug treatments ; Protein Conformation ; Proteins ; Proteins - chemistry ; Proteins - metabolism ; Proteomics ; Receptors, G-Protein-Coupled - chemistry ; Receptors, G-Protein-Coupled - metabolism ; Receptors, Vasopressin - chemistry ; Receptors, Vasopressin - metabolism ; Reproductive Biology ; Sepharose - chemistry ; Sexual reproduction</subject><ispartof>Bioorganic & medicinal chemistry letters, 2006-02, Vol.16 (3), p.521-524</ispartof><rights>2005 Elsevier Ltd</rights><rights>2006 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-5488fe5d13a5b1b912bf99eac0ec36b233f41d1d75ba1a4a33071da4b16a78693</citedby><cites>FETCH-LOGICAL-c418t-5488fe5d13a5b1b912bf99eac0ec36b233f41d1d75ba1a4a33071da4b16a78693</cites><orcidid>0000-0002-7531-4309 ; 0000-0001-9579-2503 ; 0000-0002-7380-5825 ; 0000-0003-1550-3658</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0960894X05013600$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17391033$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16289816$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-03117141$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Granier, Sébastien</creatorcontrib><creatorcontrib>Jean-Alphonse, Frédéric</creatorcontrib><creatorcontrib>Déméné, Hélène</creatorcontrib><creatorcontrib>Guillon, Gilles</creatorcontrib><creatorcontrib>Pascal, Robert</creatorcontrib><creatorcontrib>Mendre, Christiane</creatorcontrib><title>Design and synthesis of cyclic and linear peptide-agarose tools for baiting interacting protein partners of GPCRs</title><title>Bioorganic & medicinal chemistry letters</title><addtitle>Bioorg Med Chem Lett</addtitle><description>The synthesis of affinity supports designed to identify GPCR partners is reported.
A ligation strategy for the synthesis of cyclic and linear peptides covalently linked to agarose beads designed as baits to identify new interacting partners of intracellular loops of the V2 vasopressin receptor, a member of the G-protein-coupled receptor family, is reported. The peptide-resin conjugates were subsequently shown to interact specifically with a fraction of proteins present in cellular lysates.</description><subject>Amino Acid Sequence</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biochemistry, Molecular Biology</subject><subject>Biological and medical sciences</subject><subject>Cell Behavior</subject><subject>Cell Membrane - metabolism</subject><subject>Cellular Biology</subject><subject>Drug Design</subject><subject>Electrophoresis, Gel, Two-Dimensional</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>G-protein-coupled receptors</subject><subject>General aspects, investigation methods</subject><subject>Humans</subject><subject>Interactions. Associations</subject><subject>Intermolecular phenomena</subject><subject>Intracellular loop</subject><subject>Life Sciences</subject><subject>Ligation</subject><subject>Medical sciences</subject><subject>Molecular biology</subject><subject>Molecular biophysics</subject><subject>Molecular Networks</subject><subject>Molecular Sequence Data</subject><subject>Neuropharmacology</subject><subject>Neurotransmitters. Neurotransmission. Receptors</subject><subject>Peptide synthesis</subject><subject>Peptidergic system (neuropeptide, opioid peptide, opiates...). Adenosinergic and purinergic systems</subject><subject>Peptides - chemical synthesis</subject><subject>Peptides - metabolism</subject><subject>Peptides, Cyclic - chemical synthesis</subject><subject>Peptides, Cyclic - metabolism</subject><subject>Pharmaceutical sciences</subject><subject>Pharmacology</subject><subject>Pharmacology. Drug treatments</subject><subject>Protein Conformation</subject><subject>Proteins</subject><subject>Proteins - chemistry</subject><subject>Proteins - metabolism</subject><subject>Proteomics</subject><subject>Receptors, G-Protein-Coupled - chemistry</subject><subject>Receptors, G-Protein-Coupled - metabolism</subject><subject>Receptors, Vasopressin - chemistry</subject><subject>Receptors, Vasopressin - metabolism</subject><subject>Reproductive Biology</subject><subject>Sepharose - chemistry</subject><subject>Sexual reproduction</subject><issn>0960-894X</issn><issn>1464-3405</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1rGzEQhkVpady0f6CHoksDPayrWWm_oJfgNknB0FJa6E3MamcdmbV2I8kB__tqbZPcepL08uhhmJex9yCWIKD8vF22OzMscyGKFCxFCS_YAlSpMqlE8ZItRFOKrG7U3wv2JoStEKCEUq_ZBZR53dRQLtjDVwp24zi6joeDi_fpGfjYc3MwgzXHfLCO0POJpmg7ynCDfgzE4zgOgfej5y3aaN2GWxfJozneJz9Gso5P6KMjf3Te_lz9Cm_Zqx6HQO_O5yX7c_Pt9-ouW_-4_b66XmdGQR2zQtV1T0UHEosW2gbytm8aQiPIyLLNpewVdNBVRYuACqUUFXSoWiixqstGXrJPJ-89Dnrydof-oEe0-u56redMSIAKFDxCYq9ObJr6YU8h6p0NhoYBHY37oMuqSMp8luYn0KQVBE_9kxmEnkvRWz2XoudS5iyVkj59ONv37Y665y_nFhLw8QxgMDj0Hp2x4ZmrZANCysR9OXGU9vZoyetgLDlDnfVkou5G-785_gEioqry</recordid><startdate>20060201</startdate><enddate>20060201</enddate><creator>Granier, Sébastien</creator><creator>Jean-Alphonse, Frédéric</creator><creator>Déméné, Hélène</creator><creator>Guillon, Gilles</creator><creator>Pascal, Robert</creator><creator>Mendre, Christiane</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-7531-4309</orcidid><orcidid>https://orcid.org/0000-0001-9579-2503</orcidid><orcidid>https://orcid.org/0000-0002-7380-5825</orcidid><orcidid>https://orcid.org/0000-0003-1550-3658</orcidid></search><sort><creationdate>20060201</creationdate><title>Design and synthesis of cyclic and linear peptide-agarose tools for baiting interacting protein partners of GPCRs</title><author>Granier, Sébastien ; Jean-Alphonse, Frédéric ; Déméné, Hélène ; Guillon, Gilles ; Pascal, Robert ; Mendre, Christiane</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-5488fe5d13a5b1b912bf99eac0ec36b233f41d1d75ba1a4a33071da4b16a78693</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Amino Acid Sequence</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biochemistry, Molecular Biology</topic><topic>Biological and medical sciences</topic><topic>Cell Behavior</topic><topic>Cell Membrane - metabolism</topic><topic>Cellular Biology</topic><topic>Drug Design</topic><topic>Electrophoresis, Gel, Two-Dimensional</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>G-protein-coupled receptors</topic><topic>General aspects, investigation methods</topic><topic>Humans</topic><topic>Interactions. Associations</topic><topic>Intermolecular phenomena</topic><topic>Intracellular loop</topic><topic>Life Sciences</topic><topic>Ligation</topic><topic>Medical sciences</topic><topic>Molecular biology</topic><topic>Molecular biophysics</topic><topic>Molecular Networks</topic><topic>Molecular Sequence Data</topic><topic>Neuropharmacology</topic><topic>Neurotransmitters. Neurotransmission. Receptors</topic><topic>Peptide synthesis</topic><topic>Peptidergic system (neuropeptide, opioid peptide, opiates...). Adenosinergic and purinergic systems</topic><topic>Peptides - chemical synthesis</topic><topic>Peptides - metabolism</topic><topic>Peptides, Cyclic - chemical synthesis</topic><topic>Peptides, Cyclic - metabolism</topic><topic>Pharmaceutical sciences</topic><topic>Pharmacology</topic><topic>Pharmacology. Drug treatments</topic><topic>Protein Conformation</topic><topic>Proteins</topic><topic>Proteins - chemistry</topic><topic>Proteins - metabolism</topic><topic>Proteomics</topic><topic>Receptors, G-Protein-Coupled - chemistry</topic><topic>Receptors, G-Protein-Coupled - metabolism</topic><topic>Receptors, Vasopressin - chemistry</topic><topic>Receptors, Vasopressin - metabolism</topic><topic>Reproductive Biology</topic><topic>Sepharose - chemistry</topic><topic>Sexual reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Granier, Sébastien</creatorcontrib><creatorcontrib>Jean-Alphonse, Frédéric</creatorcontrib><creatorcontrib>Déméné, Hélène</creatorcontrib><creatorcontrib>Guillon, Gilles</creatorcontrib><creatorcontrib>Pascal, Robert</creatorcontrib><creatorcontrib>Mendre, Christiane</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Bioorganic & medicinal chemistry letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Granier, Sébastien</au><au>Jean-Alphonse, Frédéric</au><au>Déméné, Hélène</au><au>Guillon, Gilles</au><au>Pascal, Robert</au><au>Mendre, Christiane</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Design and synthesis of cyclic and linear peptide-agarose tools for baiting interacting protein partners of GPCRs</atitle><jtitle>Bioorganic & medicinal chemistry letters</jtitle><addtitle>Bioorg Med Chem Lett</addtitle><date>2006-02-01</date><risdate>2006</risdate><volume>16</volume><issue>3</issue><spage>521</spage><epage>524</epage><pages>521-524</pages><issn>0960-894X</issn><eissn>1464-3405</eissn><abstract>The synthesis of affinity supports designed to identify GPCR partners is reported.
A ligation strategy for the synthesis of cyclic and linear peptides covalently linked to agarose beads designed as baits to identify new interacting partners of intracellular loops of the V2 vasopressin receptor, a member of the G-protein-coupled receptor family, is reported. The peptide-resin conjugates were subsequently shown to interact specifically with a fraction of proteins present in cellular lysates.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>16289816</pmid><doi>10.1016/j.bmcl.2005.10.061</doi><tpages>4</tpages><orcidid>https://orcid.org/0000-0002-7531-4309</orcidid><orcidid>https://orcid.org/0000-0001-9579-2503</orcidid><orcidid>https://orcid.org/0000-0002-7380-5825</orcidid><orcidid>https://orcid.org/0000-0003-1550-3658</orcidid></addata></record> |
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| ispartof | Bioorganic & medicinal chemistry letters, 2006-02, Vol.16 (3), p.521-524 |
| issn | 0960-894X 1464-3405 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Amino Acid Sequence Analytical, structural and metabolic biochemistry Biochemistry, Molecular Biology Biological and medical sciences Cell Behavior Cell Membrane - metabolism Cellular Biology Drug Design Electrophoresis, Gel, Two-Dimensional Fundamental and applied biological sciences. Psychology G-protein-coupled receptors General aspects, investigation methods Humans Interactions. Associations Intermolecular phenomena Intracellular loop Life Sciences Ligation Medical sciences Molecular biology Molecular biophysics Molecular Networks Molecular Sequence Data Neuropharmacology Neurotransmitters. Neurotransmission. Receptors Peptide synthesis Peptidergic system (neuropeptide, opioid peptide, opiates...). Adenosinergic and purinergic systems Peptides - chemical synthesis Peptides - metabolism Peptides, Cyclic - chemical synthesis Peptides, Cyclic - metabolism Pharmaceutical sciences Pharmacology Pharmacology. Drug treatments Protein Conformation Proteins Proteins - chemistry Proteins - metabolism Proteomics Receptors, G-Protein-Coupled - chemistry Receptors, G-Protein-Coupled - metabolism Receptors, Vasopressin - chemistry Receptors, Vasopressin - metabolism Reproductive Biology Sepharose - chemistry Sexual reproduction |
| title | Design and synthesis of cyclic and linear peptide-agarose tools for baiting interacting protein partners of GPCRs |
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