Design and synthesis of cyclic and linear peptide-agarose tools for baiting interacting protein partners of GPCRs

The synthesis of affinity supports designed to identify GPCR partners is reported. A ligation strategy for the synthesis of cyclic and linear peptides covalently linked to agarose beads designed as baits to identify new interacting partners of intracellular loops of the V2 vasopressin receptor, a me...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Bioorganic & medicinal chemistry letters 2006-02, Vol.16 (3), p.521-524
Hauptverfasser:	Granier, Sébastien, Jean-Alphonse, Frédéric, Déméné, Hélène, Guillon, Gilles, Pascal, Robert, Mendre, Christiane
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Analytical, structural and metabolic biochemistry Biochemistry, Molecular Biology Biological and medical sciences Cell Behavior Cell Membrane - metabolism Cellular Biology Drug Design Electrophoresis, Gel, Two-Dimensional Fundamental and applied biological sciences. Psychology G-protein-coupled receptors General aspects, investigation methods Humans Interactions. Associations Intermolecular phenomena Intracellular loop Life Sciences Ligation Medical sciences Molecular biology Molecular biophysics Molecular Networks Molecular Sequence Data Neuropharmacology Neurotransmitters. Neurotransmission. Receptors Peptide synthesis Peptidergic system (neuropeptide, opioid peptide, opiates...). Adenosinergic and purinergic systems Peptides - chemical synthesis Peptides - metabolism Peptides, Cyclic - chemical synthesis Peptides, Cyclic - metabolism Pharmaceutical sciences Pharmacology Pharmacology. Drug treatments Protein Conformation Proteins Proteins - chemistry Proteins - metabolism Proteomics Receptors, G-Protein-Coupled - chemistry Receptors, G-Protein-Coupled - metabolism Receptors, Vasopressin - chemistry Receptors, Vasopressin - metabolism Reproductive Biology Sepharose - chemistry Sexual reproduction
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The synthesis of affinity supports designed to identify GPCR partners is reported. A ligation strategy for the synthesis of cyclic and linear peptides covalently linked to agarose beads designed as baits to identify new interacting partners of intracellular loops of the V2 vasopressin receptor, a member of the G-protein-coupled receptor family, is reported. The peptide-resin conjugates were subsequently shown to interact specifically with a fraction of proteins present in cellular lysates.
ISSN:	0960-894X 1464-3405
DOI:	10.1016/j.bmcl.2005.10.061