Identification of the fatty acid coenzyme‐A ligase FadD1 as an interacting partner of FptX in the Pseudomonas aeruginosa pyochelin pathway

Pseudomonas aeruginosa is one of the most important nosocomial bacteria emerging as a highly multidrug‐resistant pathogen. P. aeruginosa produces two siderophores including pyochelin (PCH) to fulfil its need for iron during infections. We know that both outer and inner membrane proteins FptA and Fpt...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:FEBS letters 2021-02, Vol.595 (3), p.370-378
Hauptverfasser: Roche, Béatrice, Mislin, Gaëtan L. A., Schalk, Isabelle J.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Pseudomonas aeruginosa is one of the most important nosocomial bacteria emerging as a highly multidrug‐resistant pathogen. P. aeruginosa produces two siderophores including pyochelin (PCH) to fulfil its need for iron during infections. We know that both outer and inner membrane proteins FptA and FptX are involved in the ferri‐PCH uptake, but this process requires increasing molecular and biochemical knowledge. Here, using bacterial two‐hybrid and copurification assays we identified the fatty acid coenzyme‐A ligase FadD1 as a novel interacting partner of the inner membrane transporter FptX and found that FadD1 may play a role in PCH production. We managed to purify the FadD1–FptX inner membrane complex and obtained low‐resolution 3D models, opening the way for future high‐resolution structures.
ISSN:0014-5793
1873-3468
DOI:10.1002/1873-3468.14012