Liposomal membrane permeability assessment by fluorescence techniques: Main permeabilizing agents, applications and challenges

[Display omitted] •Liposomes are used for membrane permeability assessment by fluorescence techniques.•Focus is laid on mechanisms underlying membrane permeabilization.•Intrinsic and extrinsic factors modulating membrane permeabilization are discussed.•The review is also helpful in the development of liposomal formulations. Liposomes are lipid vesicles made of one or multiple lipid bilayers surrounding an internal aqueous core. They are broadly employed as models to study membrane structure and properties. Among these properties, liposome membrane permeability is crucial and widely assessed by fluorescence techniques. The first part of this review is devoted to describe the various techniques used for membrane permeability assessment. Attention is paid to fluorescence techniques based on vesicle leakage of self-quenching probes, dye/quencher pair or cation/ligand pair. Secondly, the membrane-active agents inducing membrane permeabilization is presented and details on their mechanisms of action are given. Emphasis is also laid on the intrinsic and extrinsic factors that can modulate the membrane permeability. Hence, a suitable liposomal membrane should be formulated according to the aim of the study and its application.