Effects of microcarriers addition and mixing on WJ-MSC culture in bioreactors

•New strategy of controlled addition of microcarriers to improve WJ-MSC expansion.•Cell quality was not impacted by bead-to-bead transfer ability of WJ-MSC.•Orbital agitation led to a significant increase of cell death.•Mechanical agitation at Njs was more suitable for WJ-MSC expansion. Mesenchymal...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Biochemical engineering journal 2020-04, Vol.157, p.107521, Article 107521
Hauptverfasser: Sion, C., Loubière, C., Wlodarczyk-Biegun, M.K., Davoudi, N., Müller-Renno, C., Guedon, E., Chevalot, I., Olmos, E.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:•New strategy of controlled addition of microcarriers to improve WJ-MSC expansion.•Cell quality was not impacted by bead-to-bead transfer ability of WJ-MSC.•Orbital agitation led to a significant increase of cell death.•Mechanical agitation at Njs was more suitable for WJ-MSC expansion. Mesenchymal stem cells isolated from the Wharton’s jelly of human umbilical cords (WJ-MSC) are of increasing interest for cell therapies, but scalable cell production in stirred tank bioreactors (STR) still requires further investigations in order to be more efficient and with decreased costs. To handle the problem of cell confluence on microcarriers leading to cell aggregation, a new strategy of microcarriers addition was proposed. The ’bead-to-bead transfer’ ability of WJ-MSC was indeed used to maintain constant the number of cells per microcarriers. However, the resulting increase of bead shocks frequency could also negatively impact cell quantity and quality. Until now, no quantitative study describing the impact of bead interactions on WJ-MSC death was reported. In this study the influence of microcarriers addition as well as mixing characteristics on cell viability were determined. Obtained results showed that, when particle mixing is below the just-suspended state condition (Njs), local increase of particle volume fraction promotes a significant cell death in an agitation mode of orbital stirring. However, an increase in agitation rate at Njs is clearly beneficial to cell viability and growth. These effects were magnified during microcarrier addition due to the increase of mean volume fraction of particles. The present study also demonstrates the critical influence of Njs and particle distributions within the bioreactor on WJ-MSC culture performances.
ISSN:1369-703X
1873-295X
DOI:10.1016/j.bej.2020.107521