Automatic analysis of silver-stained comets by CellProfiler software

The comet assay is one of the most widely used methods to evaluate DNA damage and repair in eukaryotic cells. The comets can be measured by software, in a semi-automatic or automatic process. In this paper, we apply the CellProfiler open-source software for automatic analysis of comets from digitize...

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Veröffentlicht in:	Mutation research 2012-10, Vol.748 (1-2), p.60-64
Hauptverfasser:	González, J.E., Romero, I., Barquinero, J.F., García, O.
Format:	Artikel
Sprache:	eng
Schlagworte:	Automation CASP CellProfiler Comet assay Comet Assay - methods DNA Damage DNA repair Eukaryotes Gene expression Humans Image Processing, Computer-Assisted - methods Life Sciences Open source software Silver Staining Software
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Beschreibung
Zusammenfassung:	The comet assay is one of the most widely used methods to evaluate DNA damage and repair in eukaryotic cells. The comets can be measured by software, in a semi-automatic or automatic process. In this paper, we apply the CellProfiler open-source software for automatic analysis of comets from digitized images, reporting the percentage of tail DNA. A side-by-side comparison of CellProfiler with CASP software demonstrated good agreement between the two packages. Our work demonstrates that automatic measurement of silver-stained comets with open-source software is possible, providing significant time savings.
ISSN:	1383-5718 0027-5107 1879-3592
DOI:	10.1016/j.mrgentox.2012.06.007