Characterization and application of two RANK-specific antibodies with different biological activities

Highlights • Two RANK (TNFRSF11a)-specific monoclonal antibodies are characterized. • Both recognize human and mouse RANK with high specificity and affinity. • They are distinct by affinity and interference with receptor-ligand binding. • Human Langerhans cells highly express RANK. • The agonist ant...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Immunology letters 2016-03, Vol.171, p.5-14
Hauptverfasser:	Chypre, Mélanie, Seaman, Jonathan, Cordeiro, Olga G, Willen, Laure, Knoop, Kathryn A, Buchanan, Andrew, Sainson, Richard C.A, Williams, Ifor R, Yagita, Hideo, Schneider, Pascal, Mueller, Christopher G
Format:	Artikel
Sprache:	eng
Schlagworte:	Allergy and Immunology Animals Antibodies, Monoclonal - immunology Antibodies, Monoclonal - isolation & purification Antibody Affinity Cell Differentiation - drug effects Cell Surface Display Techniques Epithelial Cells - drug effects Epithelial Cells - physiology Epithelial microfold cell Epitopes - immunology Epitopes - metabolism HEK293 Cells Humans Immunization, Secondary Immunology Immunomodulation Intestines - cytology Intestines - drug effects Jurkat Cells Langerhans cell Langerhans Cells - immunology Life Sciences Mice Mice, Inbred C57BL Monoclonal antibody NF-kappa B - metabolism RANK (TNFRSF11a) Receptor Activator of Nuclear Factor-kappa B - immunology Receptor Activator of Nuclear Factor-kappa B - metabolism Signal Transduction
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Highlights • Two RANK (TNFRSF11a)-specific monoclonal antibodies are characterized. • Both recognize human and mouse RANK with high specificity and affinity. • They are distinct by affinity and interference with receptor-ligand binding. • Human Langerhans cells highly express RANK. • The agonist antibody stimulates intestinal microfold cells in vivo.
ISSN:	0165-2478 1879-0542
DOI:	10.1016/j.imlet.2016.01.003