Quantitative three-dimensional study on the position of the female gametophyte and its constituent cells as a prerequisite for corn (Zea mays) transformation

The position of the embryo sac in the spikelet and of the embryo sac's constituent cells within the sporophytic tissues of Zea mays was localized by scanning electron microscopy, serial thick sectioning, and computer three-dimensional reconstruction. Within certain limits, the embryo sac is con...

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Veröffentlicht in:	Theoretical and applied genetics 1990, Vol.79 (1), p.72-76
Hauptverfasser:	Wagner, V.T, Dumas, C, Mogensen, H.L
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology embryo (plant) Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics genetic transformation Genetics laboratory techniques Life Sciences Methods. Procedures. Technologies microinjection plant anatomy plant morphology ultrastructure Vectors (cloning, transfer, expression). Insertion sequences and transposons Zea mays
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Zusammenfassung:	The position of the embryo sac in the spikelet and of the embryo sac's constituent cells within the sporophytic tissues of Zea mays was localized by scanning electron microscopy, serial thick sectioning, and computer three-dimensional reconstruction. Within certain limits, the embryo sac is consistently oriented in the same position inside of the spikelet. This information is a prerequisite for successful microinjections into the in situ female cells of Zea mays.
ISSN:	0040-5752 1432-2242
DOI:	10.1007/BF00223789