Pathogenicity of Listeria monocytogenes isolates in immunocompromised mice in relation to listeriolysin production

1 Institut National de la Recherche Agronomique, Station de Pathologie de la Reproduction, 37380 Nouzilly Laboratoire de Bacteriologie, Faculte de Medecine, 37032 Tours, France * Correspondence should be sent to Dr J. De Rycke, Institut National de la Recherche Agronomique, Station de Pathologie de la Reproduction, 37380, Nouzilly, France. Received April 19, 1990 Accepted June 10, 1990 Summary. The virulence of 74 Listeria monocytogenes isolates from clinical cases and food products and of 11 isolates of other Listeria species was tested in mice immunocompromised with carrageenan. Isolates of species other than L. monocytogenes were not lethal to such mice. All 29 clinical isolates of L. monocytogenes (serotypes 1/2a, 1/2b, 4b) and 33 of 42 isolates of various serotypes isolated mainly from dairy products killed all test mice (100% lethality) at an inoculum of 10 4 cfu/mouse. All lethal strains of L. monocytogenes were haemolytic and possessed the 58-Kda band specific for listeriolysin O as demonstrated by SDS-PAGE immunoblotting. The nine avirulent strains of L. monocytogenes had detectable haemolytic activity, but in six of them this activity was significantly weaker than in virulent strains and the 58-Kda band was not detected. The other three avirulent strains were highly haemolytic and possessed the 58-Kda band, which suggests that other factor(s) could be involved in the virulence of L. monocytogenes .