Use of flow cytometry for rapid determination of ploidy level in the genus Actinidia

Measurement of ploidy levels in the genus Actinidia, using flow cytometry, was the subject of this preliminary research. Compared with other methods (chromosome counts, length of stomata), flow cytometry promises quicker and easier determination of the ploidy level. The use of this method in plants...

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Veröffentlicht in:Scientia horticulturae 1994-05, Vol.57 (4), p.303-313
Hauptverfasser: Ollitrault-Sammarcelli, Fréderique, Legave, J.M., Michaux-Ferriere, Nicole, Hirsch, Anne Marie
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container_issue 4
container_start_page 303
container_title Scientia horticulturae
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creator Ollitrault-Sammarcelli, Fréderique
Legave, J.M.
Michaux-Ferriere, Nicole
Hirsch, Anne Marie
description Measurement of ploidy levels in the genus Actinidia, using flow cytometry, was the subject of this preliminary research. Compared with other methods (chromosome counts, length of stomata), flow cytometry promises quicker and easier determination of the ploidy level. The use of this method in plants produced in vitro from seeds of ‘fruiting male’ of Actinidia deliciosa revealed the existence of individuals of high ploidy level, probably 9 ×. In general, these individuals were characterized by low vigor and an abnormally long juvenile period. Plants of this ploidy level, which to our knowledge have never before been noticed, do not seem to have any varietal potential.
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ispartof Scientia horticulturae, 1994-05, Vol.57 (4), p.303-313
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subjects ACTINIDIA
Agricultural sciences
Agronomy. Soil science and plant productions
Biological and medical sciences
CHROMOSOME NUMBER
Cytogenetics
Flow cytometry
Fundamental and applied biological sciences. Psychology
Generalities. Genetics. Plant material
Genetics and breeding of economic plants
Horticulture
In vitro culture
Life Sciences
METHODE
METHODS
METODOS
NOMBRE CHROMOSOMIQUE
NUMERO DE CROMOSOMAS
Ploidy level
title Use of flow cytometry for rapid determination of ploidy level in the genus Actinidia
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