Use of flow cytometry for rapid determination of ploidy level in the genus Actinidia
Measurement of ploidy levels in the genus Actinidia, using flow cytometry, was the subject of this preliminary research. Compared with other methods (chromosome counts, length of stomata), flow cytometry promises quicker and easier determination of the ploidy level. The use of this method in plants...
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Veröffentlicht in: | Scientia horticulturae 1994-05, Vol.57 (4), p.303-313 |
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creator | Ollitrault-Sammarcelli, Fréderique Legave, J.M. Michaux-Ferriere, Nicole Hirsch, Anne Marie |
description | Measurement of ploidy levels in the genus
Actinidia, using flow cytometry, was the subject of this preliminary research. Compared with other methods (chromosome counts, length of stomata), flow cytometry promises quicker and easier determination of the ploidy level. The use of this method in plants produced in vitro from seeds of ‘fruiting male’ of
Actinidia deliciosa revealed the existence of individuals of high ploidy level, probably 9 ×. In general, these individuals were characterized by low vigor and an abnormally long juvenile period. Plants of this ploidy level, which to our knowledge have never before been noticed, do not seem to have any varietal potential. |
doi_str_mv | 10.1016/S0304-4238(94)90113-9 |
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Actinidia deliciosa revealed the existence of individuals of high ploidy level, probably 9 ×. In general, these individuals were characterized by low vigor and an abnormally long juvenile period. Plants of this ploidy level, which to our knowledge have never before been noticed, do not seem to have any varietal potential.</description><identifier>ISSN: 0304-4238</identifier><identifier>EISSN: 1879-1018</identifier><identifier>DOI: 10.1016/S0304-4238(94)90113-9</identifier><identifier>CODEN: SHRTAH</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>ACTINIDIA ; Agricultural sciences ; Agronomy. Soil science and plant productions ; Biological and medical sciences ; CHROMOSOME NUMBER ; Cytogenetics ; Flow cytometry ; Fundamental and applied biological sciences. Psychology ; Generalities. Genetics. Plant material ; Genetics and breeding of economic plants ; Horticulture ; In vitro culture ; Life Sciences ; METHODE ; METHODS ; METODOS ; NOMBRE CHROMOSOMIQUE ; NUMERO DE CROMOSOMAS ; Ploidy level</subject><ispartof>Scientia horticulturae, 1994-05, Vol.57 (4), p.303-313</ispartof><rights>1994</rights><rights>1994 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c455t-d7f4cb01119375318950493f46803f579dc8254027883226864bfca1782b0143</citedby><cites>FETCH-LOGICAL-c455t-d7f4cb01119375318950493f46803f579dc8254027883226864bfca1782b0143</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0304-4238(94)90113-9$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,315,781,785,886,3551,27926,27927,45997</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4131074$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02701090$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Ollitrault-Sammarcelli, Fréderique</creatorcontrib><creatorcontrib>Legave, J.M.</creatorcontrib><creatorcontrib>Michaux-Ferriere, Nicole</creatorcontrib><creatorcontrib>Hirsch, Anne Marie</creatorcontrib><title>Use of flow cytometry for rapid determination of ploidy level in the genus Actinidia</title><title>Scientia horticulturae</title><description>Measurement of ploidy levels in the genus
Actinidia, using flow cytometry, was the subject of this preliminary research. Compared with other methods (chromosome counts, length of stomata), flow cytometry promises quicker and easier determination of the ploidy level. The use of this method in plants produced in vitro from seeds of ‘fruiting male’ of
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Actinidia, using flow cytometry, was the subject of this preliminary research. Compared with other methods (chromosome counts, length of stomata), flow cytometry promises quicker and easier determination of the ploidy level. The use of this method in plants produced in vitro from seeds of ‘fruiting male’ of
Actinidia deliciosa revealed the existence of individuals of high ploidy level, probably 9 ×. In general, these individuals were characterized by low vigor and an abnormally long juvenile period. Plants of this ploidy level, which to our knowledge have never before been noticed, do not seem to have any varietal potential.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><doi>10.1016/S0304-4238(94)90113-9</doi><tpages>11</tpages></addata></record> |
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source | ScienceDirect Journals (5 years ago - present) |
subjects | ACTINIDIA Agricultural sciences Agronomy. Soil science and plant productions Biological and medical sciences CHROMOSOME NUMBER Cytogenetics Flow cytometry Fundamental and applied biological sciences. Psychology Generalities. Genetics. Plant material Genetics and breeding of economic plants Horticulture In vitro culture Life Sciences METHODE METHODS METODOS NOMBRE CHROMOSOMIQUE NUMERO DE CROMOSOMAS Ploidy level |
title | Use of flow cytometry for rapid determination of ploidy level in the genus Actinidia |
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