Xanthophyll biosynthesis. Cloning, expression, functional reconstitution, and regulation of beta-cyclohexenyl carotenoid epoxidase from pepper (Capsicum annuum)

Pepper (Capsicum annuum) beta-cyclohexenyl xanthophyll epoxidase cDNA was cloned and the corresponding enzyme overexpressed and purified from Escherichia coli, for investigation of its catalytic activity. The recombinant protein did not directly accept NADPH for epoxidation of cyclohexenyl carotenoi...

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Veröffentlicht in:	The Journal of biological chemistry 1996-11, Vol.271 (46), p.28861-28867
Hauptverfasser:	Bouvier, F, d'Harlingue, A, Hugueney, P, Marin, E, Marion-Poll, A, Camara, B
Format:	Artikel
Sprache:	eng
Schlagworte:	Amines - metabolism Amino Acid Sequence Base Sequence Biochemistry Biochemistry, Molecular Biology Capsicum annuum Cloning, Molecular DNA, Complementary Escherichia coli Escherichia coli - genetics Life Sciences Lutein - biosynthesis Molecular Sequence Data Oxidoreductases - genetics Oxidoreductases - metabolism Plastids Sequence Homology, Amino Acid Vegetables - enzymology
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