Repeated sequence RSα is diagnostic for Bradyrhizobium japonicum and Bradyrhizobium elkanii
The genome of Bradyrhizobium japonicum and B. elkanii contains multiple copies of the repeated DNA sequence RSα. A collection of 18 B. japonicum, 4 B. elkanii and 72 other bacterial strains was screened by polymerase chain reaction (PCR) using a pair of primers specific for RSα. Only strains of B. j...
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Veröffentlicht in: | Biology and fertility of soils 1996, Vol.23 (1), p.15-19 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | The genome of Bradyrhizobium japonicum and B. elkanii contains multiple copies of the repeated DNA sequence RSα. A collection of 18 B. japonicum, 4 B. elkanii and 72 other bacterial strains was screened by polymerase chain reaction (PCR) using a pair of primers specific for RSα. Only strains of B. japonicum and B. elkanii gave the predicted amplification product. Restriction analysis of PCR products obtained from different strains of B. japonicum showed that the RSα sequence was generally conserved. The usefulness of RSα as a specific probe for Bradyrhizobium strains capable of nodulating soybean was also demonstrated. |
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ISSN: | 0178-2762 1432-0789 |
DOI: | 10.1007/BF00335812 |