Rapid analysis of ergovaline in ovine plasma using high-performance liquid chromatography with fluorimetric detection
A rapid high-performance liquid chromatographic method for the determination of the mycotoxin ergovaline in ovine plasma is described here. Ergotamine was used as an internal standard. A simple extraction procedure with diethyloxide was carried out, before chromatography on a C 8 column, with the ex...
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| Veröffentlicht in: | Journal of Chromatography A 1998-07, Vol.815 (1), p.147-153 |
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| creator | Jaussaud, Philippe Durix, Andrée Videmann, Bernadette Vigié, Alain Bony, Sylvie |
| description | A rapid high-performance liquid chromatographic method for the determination of the mycotoxin ergovaline in ovine plasma is described here. Ergotamine was used as an internal standard. A simple extraction procedure with diethyloxide was carried out, before chromatography on a C
8 column, with the excitation and emission wavelengths fixed at 250 and 420 nm respectively, on a fluorimetric detector. The method, which was found to be linear between 3.5 and 15 ng/ml, had good specificity, precision and accuracy. The limit of quantification and the limit of detection were 3.5 and 1.2 ng/ml, respectively. A preliminary application of the described assay to a plasma kinetic study, after intravenous administration of a single dose of ergovaline (17 μg/kg body mass) to four sheep, showed a very rapid decrease of the plasma ergovaline levels. The terminal half-life and the total clearance of the mycotoxin were found to be 23.6 min and 0.020 l/min kg
−1 body mass, respectively. |
| doi_str_mv | 10.1016/S0021-9673(98)00002-8 |
| format | Article |
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8 column, with the excitation and emission wavelengths fixed at 250 and 420 nm respectively, on a fluorimetric detector. The method, which was found to be linear between 3.5 and 15 ng/ml, had good specificity, precision and accuracy. The limit of quantification and the limit of detection were 3.5 and 1.2 ng/ml, respectively. A preliminary application of the described assay to a plasma kinetic study, after intravenous administration of a single dose of ergovaline (17 μg/kg body mass) to four sheep, showed a very rapid decrease of the plasma ergovaline levels. The terminal half-life and the total clearance of the mycotoxin were found to be 23.6 min and 0.020 l/min kg
−1 body mass, respectively.</description><subject>Animals</subject><subject>Area Under Curve</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Ergotamine</subject><subject>Ergotamines - blood</subject><subject>Ergotamines - pharmacokinetics</subject><subject>Ergovaline</subject><subject>Half-Life</subject><subject>Indicators and Reagents</subject><subject>Injections, Intravenous</subject><subject>Life Sciences</subject><subject>Male</subject><subject>Mycotoxins</subject><subject>Sheep</subject><subject>Spectrometry, Fluorescence</subject><subject>Spectrophotometry, Ultraviolet</subject><subject>Toxins</subject><issn>0021-9673</issn><issn>1873-3778</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV9r2zAUxcXYaLNsH6Ggp7I-uNMfW5afRgntWggMtvZZ3EhyrCFbrmSn5NtPaUJepxeJe3_3XHEOQleU3FJCxfc_hDBaNKLm3xp5Q_JhhfyAFlTWvOB1LT-ixRm5RJ9T-ksIrUnNLtBFU2eMVgs0_4bRGQwD-H1yCYcW27gNO_BusNgNOOwOj9FD6gHPyQ1b3LltV4w2tiH2MGiLvXuds4juYuhhCtsIY7fHb27qcOvnEF1vp-g0NnayenJh-II-teCT_Xq6l-jl4f559Visf_18Wt2tC82bcipYJapKbwQ1DHTLgDdmA1UpCW2AaQ6llIxo3ooMgtjwstSWciINMKZpa_gS3Rx1O_BqzP-AuFcBnHq8W6tDjTAhJefVjmb2-siOMbzONk2qd0lb72GwYU6q5lKUIsNLVB1BHUNK0bZnZUrUIRr1Ho06-K4aqd6jUTLPXZ0WzJvemvPUKYvc_3Hs2-zIztmoknY2-2tczLYpE9x_NvwDb8Kfqg</recordid><startdate>19980731</startdate><enddate>19980731</enddate><creator>Jaussaud, Philippe</creator><creator>Durix, Andrée</creator><creator>Videmann, Bernadette</creator><creator>Vigié, Alain</creator><creator>Bony, Sylvie</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope></search><sort><creationdate>19980731</creationdate><title>Rapid analysis of ergovaline in ovine plasma using high-performance liquid chromatography with fluorimetric detection</title><author>Jaussaud, Philippe ; Durix, Andrée ; Videmann, Bernadette ; Vigié, Alain ; Bony, Sylvie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c394t-25655cb61d2acf2a39dba548019a2c3a48820c3f6256a6b344ce1308da22c1fd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>Area Under Curve</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Ergotamine</topic><topic>Ergotamines - blood</topic><topic>Ergotamines - pharmacokinetics</topic><topic>Ergovaline</topic><topic>Half-Life</topic><topic>Indicators and Reagents</topic><topic>Injections, Intravenous</topic><topic>Life Sciences</topic><topic>Male</topic><topic>Mycotoxins</topic><topic>Sheep</topic><topic>Spectrometry, Fluorescence</topic><topic>Spectrophotometry, Ultraviolet</topic><topic>Toxins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jaussaud, Philippe</creatorcontrib><creatorcontrib>Durix, Andrée</creatorcontrib><creatorcontrib>Videmann, Bernadette</creatorcontrib><creatorcontrib>Vigié, Alain</creatorcontrib><creatorcontrib>Bony, Sylvie</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Journal of Chromatography A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jaussaud, Philippe</au><au>Durix, Andrée</au><au>Videmann, Bernadette</au><au>Vigié, Alain</au><au>Bony, Sylvie</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid analysis of ergovaline in ovine plasma using high-performance liquid chromatography with fluorimetric detection</atitle><jtitle>Journal of Chromatography A</jtitle><addtitle>J Chromatogr A</addtitle><date>1998-07-31</date><risdate>1998</risdate><volume>815</volume><issue>1</issue><spage>147</spage><epage>153</epage><pages>147-153</pages><issn>0021-9673</issn><eissn>1873-3778</eissn><abstract>A rapid high-performance liquid chromatographic method for the determination of the mycotoxin ergovaline in ovine plasma is described here. Ergotamine was used as an internal standard. A simple extraction procedure with diethyloxide was carried out, before chromatography on a C
8 column, with the excitation and emission wavelengths fixed at 250 and 420 nm respectively, on a fluorimetric detector. The method, which was found to be linear between 3.5 and 15 ng/ml, had good specificity, precision and accuracy. The limit of quantification and the limit of detection were 3.5 and 1.2 ng/ml, respectively. A preliminary application of the described assay to a plasma kinetic study, after intravenous administration of a single dose of ergovaline (17 μg/kg body mass) to four sheep, showed a very rapid decrease of the plasma ergovaline levels. The terminal half-life and the total clearance of the mycotoxin were found to be 23.6 min and 0.020 l/min kg
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Area Under Curve Chromatography, High Pressure Liquid Ergotamine Ergotamines - blood Ergotamines - pharmacokinetics Ergovaline Half-Life Indicators and Reagents Injections, Intravenous Life Sciences Male Mycotoxins Sheep Spectrometry, Fluorescence Spectrophotometry, Ultraviolet Toxins |
| title | Rapid analysis of ergovaline in ovine plasma using high-performance liquid chromatography with fluorimetric detection |
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