Rapid analysis of ergovaline in ovine plasma using high-performance liquid chromatography with fluorimetric detection
A rapid high-performance liquid chromatographic method for the determination of the mycotoxin ergovaline in ovine plasma is described here. Ergotamine was used as an internal standard. A simple extraction procedure with diethyloxide was carried out, before chromatography on a C 8 column, with the ex...
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Veröffentlicht in: | Journal of Chromatography A 1998-07, Vol.815 (1), p.147-153 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | A rapid high-performance liquid chromatographic method for the determination of the mycotoxin ergovaline in ovine plasma is described here. Ergotamine was used as an internal standard. A simple extraction procedure with diethyloxide was carried out, before chromatography on a C
8 column, with the excitation and emission wavelengths fixed at 250 and 420 nm respectively, on a fluorimetric detector. The method, which was found to be linear between 3.5 and 15 ng/ml, had good specificity, precision and accuracy. The limit of quantification and the limit of detection were 3.5 and 1.2 ng/ml, respectively. A preliminary application of the described assay to a plasma kinetic study, after intravenous administration of a single dose of ergovaline (17 μg/kg body mass) to four sheep, showed a very rapid decrease of the plasma ergovaline levels. The terminal half-life and the total clearance of the mycotoxin were found to be 23.6 min and 0.020 l/min kg
−1 body mass, respectively. |
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ISSN: | 0021-9673 1873-3778 |
DOI: | 10.1016/S0021-9673(98)00002-8 |