Mice immunization with live lactococci displaying a surface anchored HPV-16 E7 oncoprotein

E7 oncoprotein of human papillomavirus-16 (HPV-16) is constitutively produced in cervical cancer (CxCa) and is a good candidate for the design of therapeutic vaccines. In this work, the nisin-controlled expression system was used to display the E7 protein at the cell surface of the food-grade Gram-p...

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Veröffentlicht in:	FEMS microbiology letters 2003-12, Vol.229 (1), p.37-42
Hauptverfasser:	Cortes-Perez, Naima G, Bermúdez-Humarán, Luis G, Le Loir, Yves, Rodriguez-Padilla, Cristina, Gruss, Alexandra, Saucedo-Cárdenas, Odila, Langella, Philippe, Montes-de-Oca-Luna, Roberto
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antigens, Surface - immunology Applied microbiology Biological and medical sciences Cancer Cell Wall - immunology Female Fundamental and applied biological sciences. Psychology Human health and pathology Human papillomavirus Human papillomavirus 16 Immunization - methods Immunogenic Lactococcus - immunology Lactococcus lactis Life Sciences Mice Mice, Inbred C57BL Microbiology Microbiology and Parasitology Oncogene Proteins, Viral - immunology Papillomavirus E7 Proteins Uterine Cervical Neoplasms - immunology Uterine Cervical Neoplasms - prevention & control Vaccine Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Virology
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Zusammenfassung:	E7 oncoprotein of human papillomavirus-16 (HPV-16) is constitutively produced in cervical cancer (CxCa) and is a good candidate for the design of therapeutic vaccines. In this work, the nisin-controlled expression system was used to display the E7 protein at the cell surface of the food-grade Gram-positive bacterium Lactococcus lactis. An efficient cell wall anchoring of E7 was obtained. Intranasal administration of these recombinant lactococci in mice induced an HPV-16 E7-specific immune response. This is the first report of E7 cell wall anchoring in L. lactis and represents one more step towards the use of live food-grade bacteria to fight against CxCa.
ISSN:	0378-1097 1574-6968
DOI:	10.1016/S0378-1097(03)00778-X