Isolation and characterization of microsatellite markers in the tropical tree species Dicorynia guianensis (Caesalpinaceae)

Dicorynia guianensis is a canopy tree endemic to French Guyana and the first to be exploited as timber wood. To investigate levels of diversity and dynamics of gene flow in a 40‐ha study plot, highly polymorphic microsatellite markers were developed. An enriched microsatellite library was constructe...

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Veröffentlicht in:Molecular ecology notes 2002-09, Vol.2 (3), p.228-230
Hauptverfasser: Latouche-Hallé, C., Ramboer, A., Bandou, E., Caron, H., Decroocq, S.
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Sprache:eng
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Zusammenfassung:Dicorynia guianensis is a canopy tree endemic to French Guyana and the first to be exploited as timber wood. To investigate levels of diversity and dynamics of gene flow in a 40‐ha study plot, highly polymorphic microsatellite markers were developed. An enriched microsatellite library was constructed and seven microsatellite primer pairs were developed. Total genomic DNA was extracted and amplified from cambium tissue from 172 adult individuals. The level of genetic diversity was found to be low. A large heterozygote deficiency was found at one locus. This was resolved by redesigning the primer set.
ISSN:1471-8278
1471-8286
DOI:10.1046/j.1471-8286.2002.00196.x