Changes in adipocyte hormones and lipid oxidation associated with weight loss and regain in severely obese adolescents

OBJECTIVE: To investigate changes in adipocyte hormones and lipid oxidation during and after a weight-reduction programme in severely obese adolescents. DESIGN: Longitudinal-clinical investigation including a 9-month multidisciplinary weight-reduction programme in a specialised institution with lifestyle education, moderate energy restriction and regular physical activity, followed by a 4-month period at home. SUBJECTS: A total of 26 (12 boys and 14 girls) severely obese adolescents (mean BMI: 33.9 kg/m 2 ; 41.5% fat mass (FM)). MEASUREMENTS: Before starting (M0), at the end (M9), and 4 months after the end (M13) of the intervention blood samples were collected at fast, body composition was assessed by dual X-ray absorptiometry, and energy expenditure (EE) and substrate oxidation were assessed by whole-body indirect calorimetry over 24 h. RESULTS: At M9, adolescents had lost 19.0% body weight (BW), 41.3% FM ( P