A comparison of the internal transcribed spacer of the ribosomal DNA for Eubothrium crassum and Eubothrium salvelini (Cestoda: Pseudophyllidea), parasites of salmonid fish
The internal transcribed spacers (ITS-1 and ITS-2) and 5.8S subunit of the ribosomal RNA gene of Eubothrium crassum and Eubothrium salvelini, cestode parasites of salmonid fish, were sequenced and compared. The lengths and GC contents of the regions sequenced were 1119 bp and 50.1% for E. crassum, a...
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| Veröffentlicht in: | International journal for parasitology 2001, Vol.31 (1), p.93-96 |
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| creator | Král’ová, I. Hanzelová, V. Scholz, T. Gerdeaux, D. Špakulová, M. |
| description | The internal transcribed spacers (ITS-1 and ITS-2) and 5.8S subunit of the ribosomal RNA gene of
Eubothrium crassum and
Eubothrium salvelini, cestode parasites of salmonid fish, were sequenced and compared. The lengths and GC contents of the regions sequenced were 1119 bp and 50.1% for
E. crassum, and 1117 bp and 50.5% for
E. salvelini, respectively. Sequence alignment and comparison of both taxa showed 97.9% similarity. Within the ITS-1 and ITS-2 of both species, the restriction enzymes
MspI and
Bsh1236I possessed different cleavage sites and could be employed for rapid differentiation of both species of
Eubothrium by PCR-RFLP. |
| doi_str_mv | 10.1016/S0020-7519(00)00147-8 |
| format | Article |
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Eubothrium crassum and
Eubothrium salvelini, cestode parasites of salmonid fish, were sequenced and compared. The lengths and GC contents of the regions sequenced were 1119 bp and 50.1% for
E. crassum, and 1117 bp and 50.5% for
E. salvelini, respectively. Sequence alignment and comparison of both taxa showed 97.9% similarity. Within the ITS-1 and ITS-2 of both species, the restriction enzymes
MspI and
Bsh1236I possessed different cleavage sites and could be employed for rapid differentiation of both species of
Eubothrium by PCR-RFLP.</description><identifier>ISSN: 0020-7519</identifier><identifier>EISSN: 1879-0135</identifier><identifier>DOI: 10.1016/S0020-7519(00)00147-8</identifier><identifier>PMID: 11165277</identifier><identifier>CODEN: IJPYBT</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Amino Acid Sequence ; Animals ; Base Sequence ; Biological and medical sciences ; Brackish ; Cestoda ; Cestoda - classification ; Cestoda - genetics ; Deoxyribonuclease HpaII - metabolism ; DNA, Ribosomal Spacer - chemistry ; DNA, Ribosomal Spacer - genetics ; Eubothrium crassum ; Eubothrium salvelini ; Freshwater ; Fundamental and applied biological sciences. Psychology ; Internal transcribed spacer ; Interspecific comparison ; Intraspecific variability ; Invertebrates ; Life Sciences ; Marine ; Microbiology and Parasitology ; Molecular Sequence Data ; Nemathelminthia. Plathelmintha ; PCR-RFLP ; Phylogeny ; Polymerase Chain Reaction - veterinary ; Polymorphism, Restriction Fragment Length ; Pseudophyllidea ; Restriction Mapping - veterinary ; RNA, Ribosomal, 5.8S - genetics ; rRNA ; rRNA 5.8S ; Salmonidae ; Salmonidae - parasitology ; Sequence Alignment - veterinary ; Systematics. Geographical distribution</subject><ispartof>International journal for parasitology, 2001, Vol.31 (1), p.93-96</ispartof><rights>2001 Australian Society for Parasitology Inc</rights><rights>2001 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c549t-8feee8d2140352c97bc044398db827eda28548f9f176aaa1f5600efdea18dbac3</citedby><cites>FETCH-LOGICAL-c549t-8feee8d2140352c97bc044398db827eda28548f9f176aaa1f5600efdea18dbac3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0020751900001478$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,4010,27900,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=985564$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11165277$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02672346$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Král’ová, I.</creatorcontrib><creatorcontrib>Hanzelová, V.</creatorcontrib><creatorcontrib>Scholz, T.</creatorcontrib><creatorcontrib>Gerdeaux, D.</creatorcontrib><creatorcontrib>Špakulová, M.</creatorcontrib><title>A comparison of the internal transcribed spacer of the ribosomal DNA for Eubothrium crassum and Eubothrium salvelini (Cestoda: Pseudophyllidea), parasites of salmonid fish</title><title>International journal for parasitology</title><addtitle>Int J Parasitol</addtitle><description>The internal transcribed spacers (ITS-1 and ITS-2) and 5.8S subunit of the ribosomal RNA gene of
Eubothrium crassum and
Eubothrium salvelini, cestode parasites of salmonid fish, were sequenced and compared. The lengths and GC contents of the regions sequenced were 1119 bp and 50.1% for
E. crassum, and 1117 bp and 50.5% for
E. salvelini, respectively. Sequence alignment and comparison of both taxa showed 97.9% similarity. Within the ITS-1 and ITS-2 of both species, the restriction enzymes
MspI and
Bsh1236I possessed different cleavage sites and could be employed for rapid differentiation of both species of
Eubothrium by PCR-RFLP.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Brackish</subject><subject>Cestoda</subject><subject>Cestoda - classification</subject><subject>Cestoda - genetics</subject><subject>Deoxyribonuclease HpaII - metabolism</subject><subject>DNA, Ribosomal Spacer - chemistry</subject><subject>DNA, Ribosomal Spacer - genetics</subject><subject>Eubothrium crassum</subject><subject>Eubothrium salvelini</subject><subject>Freshwater</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Internal transcribed spacer</subject><subject>Interspecific comparison</subject><subject>Intraspecific variability</subject><subject>Invertebrates</subject><subject>Life Sciences</subject><subject>Marine</subject><subject>Microbiology and Parasitology</subject><subject>Molecular Sequence Data</subject><subject>Nemathelminthia. Plathelmintha</subject><subject>PCR-RFLP</subject><subject>Phylogeny</subject><subject>Polymerase Chain Reaction - veterinary</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Pseudophyllidea</subject><subject>Restriction Mapping - veterinary</subject><subject>RNA, Ribosomal, 5.8S - genetics</subject><subject>rRNA</subject><subject>rRNA 5.8S</subject><subject>Salmonidae</subject><subject>Salmonidae - parasitology</subject><subject>Sequence Alignment - veterinary</subject><subject>Systematics. Geographical distribution</subject><issn>0020-7519</issn><issn>1879-0135</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0VGLEzEQB_BFFK-efgQlIMgVXE12k03WFyn19ISigvocpsmERnY3vWS3cJ_pvuSl11p9u6fA8JvMJP-ieMnoO0ZZ8_4npRUtpWDtBaVzShmXpXpUzJiSbUlZLR4XsxM5K56l9CcjUXP-tDhjjDWiknJW3C6ICf0Wok9hIMGRcYPEDyPGAToyRhiSiX6NlqQtGIx_Sa6FFPpsPn1bEBciuZzWYdxEP_XEREgpnzDY_8sJuh12fvDkYolpDBY-kB8JJxu2m5uu8xZh_pbkXSD5EdN-VG7pw-AtcT5tnhdPHHQJXxzP8-L358tfy6ty9f3L1-ViVRrB27FUDhGVrRintahMK9eGcl63yq5VJdFCpQRXrnVMNgDAnGgoRZens0zA1OfF_HDvBjq9jb6HeKMDeH21WOl9jVaNrGre7Fi2bw52G8P1lF-le58Mdh0MGKakJW2qthX8Qcik4lS0MkNxgCaGlCK60wqM6n30-j56vc9VU6rvo9cq9706DpjWPdp_XcesM3h9BJAMdC5na3w6uVYJ0ez3_HhQmH945zHqZDwOBq2PaEZtg39gkTtYRszl</recordid><startdate>2001</startdate><enddate>2001</enddate><creator>Král’ová, I.</creator><creator>Hanzelová, V.</creator><creator>Scholz, T.</creator><creator>Gerdeaux, D.</creator><creator>Špakulová, M.</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H98</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>P64</scope><scope>7X8</scope><scope>1XC</scope></search><sort><creationdate>2001</creationdate><title>A comparison of the internal transcribed spacer of the ribosomal DNA for Eubothrium crassum and Eubothrium salvelini (Cestoda: Pseudophyllidea), parasites of salmonid fish</title><author>Král’ová, I. ; Hanzelová, V. ; Scholz, T. ; Gerdeaux, D. ; Špakulová, M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c549t-8feee8d2140352c97bc044398db827eda28548f9f176aaa1f5600efdea18dbac3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Brackish</topic><topic>Cestoda</topic><topic>Cestoda - classification</topic><topic>Cestoda - genetics</topic><topic>Deoxyribonuclease HpaII - metabolism</topic><topic>DNA, Ribosomal Spacer - chemistry</topic><topic>DNA, Ribosomal Spacer - genetics</topic><topic>Eubothrium crassum</topic><topic>Eubothrium salvelini</topic><topic>Freshwater</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Internal transcribed spacer</topic><topic>Interspecific comparison</topic><topic>Intraspecific variability</topic><topic>Invertebrates</topic><topic>Life Sciences</topic><topic>Marine</topic><topic>Microbiology and Parasitology</topic><topic>Molecular Sequence Data</topic><topic>Nemathelminthia. Plathelmintha</topic><topic>PCR-RFLP</topic><topic>Phylogeny</topic><topic>Polymerase Chain Reaction - veterinary</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Pseudophyllidea</topic><topic>Restriction Mapping - veterinary</topic><topic>RNA, Ribosomal, 5.8S - genetics</topic><topic>rRNA</topic><topic>rRNA 5.8S</topic><topic>Salmonidae</topic><topic>Salmonidae - parasitology</topic><topic>Sequence Alignment - veterinary</topic><topic>Systematics. Geographical distribution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Král’ová, I.</creatorcontrib><creatorcontrib>Hanzelová, V.</creatorcontrib><creatorcontrib>Scholz, T.</creatorcontrib><creatorcontrib>Gerdeaux, D.</creatorcontrib><creatorcontrib>Špakulová, M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>International journal for parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Král’ová, I.</au><au>Hanzelová, V.</au><au>Scholz, T.</au><au>Gerdeaux, D.</au><au>Špakulová, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A comparison of the internal transcribed spacer of the ribosomal DNA for Eubothrium crassum and Eubothrium salvelini (Cestoda: Pseudophyllidea), parasites of salmonid fish</atitle><jtitle>International journal for parasitology</jtitle><addtitle>Int J Parasitol</addtitle><date>2001</date><risdate>2001</risdate><volume>31</volume><issue>1</issue><spage>93</spage><epage>96</epage><pages>93-96</pages><issn>0020-7519</issn><eissn>1879-0135</eissn><coden>IJPYBT</coden><abstract>The internal transcribed spacers (ITS-1 and ITS-2) and 5.8S subunit of the ribosomal RNA gene of
Eubothrium crassum and
Eubothrium salvelini, cestode parasites of salmonid fish, were sequenced and compared. The lengths and GC contents of the regions sequenced were 1119 bp and 50.1% for
E. crassum, and 1117 bp and 50.5% for
E. salvelini, respectively. Sequence alignment and comparison of both taxa showed 97.9% similarity. Within the ITS-1 and ITS-2 of both species, the restriction enzymes
MspI and
Bsh1236I possessed different cleavage sites and could be employed for rapid differentiation of both species of
Eubothrium by PCR-RFLP.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>11165277</pmid><doi>10.1016/S0020-7519(00)00147-8</doi><tpages>4</tpages></addata></record> |
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| ispartof | International journal for parasitology, 2001, Vol.31 (1), p.93-96 |
| issn | 0020-7519 1879-0135 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Amino Acid Sequence Animals Base Sequence Biological and medical sciences Brackish Cestoda Cestoda - classification Cestoda - genetics Deoxyribonuclease HpaII - metabolism DNA, Ribosomal Spacer - chemistry DNA, Ribosomal Spacer - genetics Eubothrium crassum Eubothrium salvelini Freshwater Fundamental and applied biological sciences. Psychology Internal transcribed spacer Interspecific comparison Intraspecific variability Invertebrates Life Sciences Marine Microbiology and Parasitology Molecular Sequence Data Nemathelminthia. Plathelmintha PCR-RFLP Phylogeny Polymerase Chain Reaction - veterinary Polymorphism, Restriction Fragment Length Pseudophyllidea Restriction Mapping - veterinary RNA, Ribosomal, 5.8S - genetics rRNA rRNA 5.8S Salmonidae Salmonidae - parasitology Sequence Alignment - veterinary Systematics. Geographical distribution |
| title | A comparison of the internal transcribed spacer of the ribosomal DNA for Eubothrium crassum and Eubothrium salvelini (Cestoda: Pseudophyllidea), parasites of salmonid fish |
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